a 2014

Exploring the cystatins of helminths

ILGOVÁ, Jana, Břetislav KOUDELA, Milan GELNAR and Martin KAŠNÝ

Basic information

Original name

Exploring the cystatins of helminths

Authors

ILGOVÁ, Jana (703 Slovakia, guarantor, belonging to the institution), Břetislav KOUDELA (203 Czech Republic), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

V4 Parasitological meeting, 2014

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/14:00073668

Organization unit

Faculty of Science

ISBN

978-80-968473-7-2

Keywords in English

Eudiplozoon nipponicum; Hymenolepis diminuta; Fascioloides magna; Trichinella spiralis; cystatin; inhibitor; PCR RACE; coding sequence
Změněno: 5/6/2014 12:00, Mgr. et Mgr. Jana Ilgová, Ph.D.

Abstract

V originále

Parasitic organisms release numerous types of molecules essential for their survival. Cystatins - cysteine peptidase inhibitors (CPI) were determined as important protein molecules which participate in the processes in the host-parasite interface. Their effect can influence the host immune response, particularly the processing and presentation of antigens, production of nitric oxide, etc. The aim of our study is to expand the information about cystatins produced by helminths and to reveal their functions in four selected parasitic model species representing the major groups of helminths. We used bioinformatic tools to analyze the transcriptomic and/or genomic data of Eudiplozoon nipponicum (Monogenea), Hymenolepis diminuta (Cestoda), Fascioloides magna (Trematoda) and Trichinella spiralis (Nematoda) and we searched for the presence of conserved cystatin domains. The selected genes of CPI of diverse families - stefins, cystatins and kininogens were amplified, cloned and sequenced and significant differences in their nucleotide/amino acid primary structure were revealed. The production of recombinant forms of CPI is in process in order to characterize their structures and functions.

Links

GAP506/12/1258, research and development project
Name: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu
Investor: Czech Science Foundation
GBP505/12/G112, research and development project
Name: ECIP - Evropské centrum ichtyoparazitologie
Investor: Czech Science Foundation
MUNI/A/0888/2013, interní kód MU
Name: Biodiverzita - analýzy biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA3)
Investor: Masaryk University, Category A