Piezoelectric biosensors for detection of microorganisms

FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL. Piezoelectric biosensors for detection of microorganisms. Online. In Libuše Trnková. XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků. Vydání první. Brno: Masarykova univerzita, 2014, p. 32-35. ISBN 978-80-210-6842-1.

Basic information

• Original name: Piezoelectric biosensors for detection of microorganisms
• Authors: FARKA, Zdeněk (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Vydání první. Brno, XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků, p. 32-35, 4 pp. 2014.
• Publisher: Masarykova univerzita

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Publication form: electronic version available online
• WWW: URL
• RIV identification code: RIV/00216224:14740/14:00075645
• Organization unit: Central European Institute of Technology
• ISBN: 978-80-210-6842-1
• Keywords in English: Biosensor; Escherichia coli; Immunosensor; QCM
• Tags: kontrola MP, rivok, WOS

Abstract

Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor was applied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU·mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection of microorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved LOD was 1.45·10^4 CFU·L-1 of air and the time from sample collection to detection was 16 min.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology