Piezoelectric biosensors for detection of microorganisms

D 2014

Piezoelectric biosensors for detection of microorganisms

FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL

Basic information

Original name

Piezoelectric biosensors for detection of microorganisms

Authors

FARKA, Zdeněk (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Vydání první. Brno, XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků, p. 32-35, 4 pp. 2014

Publisher

Masarykova univerzita

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14740/14:00075645

Organization unit

Central European Institute of Technology

ISBN

978-80-210-6842-1

Keywords in English

Biosensor; Escherichia coli; Immunosensor; QCM

Abstract

V originále

Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor was applied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU·mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection of microorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved LOD was 1.45·10^4 CFU·L-1 of air and the time from sample collection to detection was 16 min.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL. Piezoelectric biosensors for detection of microorganisms. Online. In Libuše Trnková. XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků. Vydání první. Brno: Masarykova univerzita, 2014, p. 32-35. ISBN 978-80-210-6842-1.

@inproceedings{1184846,
   author = {Farka, Zdeněk and Kovář, David and Skládal, Petr},
   address = {Brno},
   booktitle = {XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků},
   edition = {Vydání první},
   editor = {Libuše Trnková},
   keywords = {Biosensor; Escherichia coli; Immunosensor; QCM},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-6842-1},
   pages = {32-35},
   publisher = {Masarykova univerzita},
   title = {Piezoelectric biosensors for detection of microorganisms},
   url = {http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34},
   year = {2014}
}

TY  - JOUR
ID  - 1184846
AU  - Farka, Zdeněk - Kovář, David - Skládal, Petr
PY  - 2014
TI  - Piezoelectric biosensors for detection of microorganisms
PB  - Masarykova univerzita
CY  - Brno
SN  - 9788021068421
KW  - Biosensor
KW  - Escherichia coli
KW  - Immunosensor
KW  - QCM
UR  - http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34
L2  - http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34
N2  - Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor was applied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU·mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection of microorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved LOD was 1.45·10^4 CFU·L-1 of air and the time from sample collection to detection was 16 min.
ER  -

FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL. Piezoelectric biosensors for detection of microorganisms. Online. In Libuše Trnková. \textit{XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků}. Vydání první. Brno: Masarykova univerzita, 2014, p.~32-35. ISBN~978-80-210-6842-1.

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