Recombinant antibodies for in vivo applications

ZÁBRADY, Matej. Recombinant antibodies for in vivo applications. In XII Discussions in Structural Molecular Biology. 2014.

Basic information

• Original name: Recombinant antibodies for in vivo applications
• Authors: ZÁBRADY, Matej (703 Slovakia, guarantor, belonging to the institution).
• Edition: XII Discussions in Structural Molecular Biology, 2014.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: XII Discussions in Structural Molecular Biology
• RIV identification code: RIV/00216224:14310/14:00073670
• Organization unit: Faculty of Science
• Keywords in English: recombinant antibodies in vivo inhibition yeast two=hybrid

Abstract

Interactions between biomolecules constitute the core of biological functions. In protein–protein interactions, specificity plays a key role and many proteins have evolved into extraordinarily precise molecular machines. The multistep phosphorylation pathway of the cytokinin signaling in plants is involving promiscuous protein-protein interactions, allowing the plasticity of the plant cell response to environmental and developmental stimuli. We have developed a recombinant antibody which is able to precisely bind to a specific protein from the phosporylation pathway in vivo. Hereinafter this protein is not phosphorylated after the signal perception and therefore the message is not delivered to the nucleus to commit the reaction of the plant. To accomplish a successful selection of the recombinant antibody, we have developed a in vivo selection method. This method allows targeting of recombinant antibodies for a precise activity. Along the way of developing our method, we came to several conclusions. In vivo selection process is important, because the stability of the recombinant antibody in the reducing environment of the plant cell is difficult to predict. Additionally, the uncertainty of the correct form of the antigen produced by heterologous expression is introducing another variable, which might result in the unsuccessful selection of a correct recombinant antibody.

Links

• GA521/09/1699, research and development project: Name: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana

Investor: Czech Science Foundation, Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana