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@article{1185430,
author = {Nechvátal, Lukáš and Pětrošová, Helena and Grillová, Linda and Pospíšilová, Petra and Mikalová, Lenka and Strnadel, Radim and Kuklová, Ivana and Kojanová, Martina and Kreidlová, Miluše and Vaňousová, Daniela and Procházka, Přemysl and Zákoucká, Hana and Krchňáková, Alena and Šmajs, David},
article_location = {JENA},
article_number = {5-6},
doi = {http://dx.doi.org/10.1016/j.ijmm.2014.04.007},
keywords = {Treponema pallidum; Syphilis; Yaws; Molecular typing; Multilocus analysis; SS14-like strains},
language = {eng},
issn = {1438-4221},
journal = {International Journal of Medical Microbiology},
title = {Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains},
volume = {304},
year = {2014}
}
TY - JOUR
ID - 1185430
AU - Nechvátal, Lukáš - Pětrošová, Helena - Grillová, Linda - Pospíšilová, Petra - Mikalová, Lenka - Strnadel, Radim - Kuklová, Ivana - Kojanová, Martina - Kreidlová, Miluše - Vaňousová, Daniela - Procházka, Přemysl - Zákoucká, Hana - Krchňáková, Alena - Šmajs, David
PY - 2014
TI - Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains
JF - International Journal of Medical Microbiology
VL - 304
IS - 5-6
SP - 645-653
EP - 645-653
PB - Urban & Fischer Verlag
SN - 14384221
KW - Treponema pallidum
KW - Syphilis
KW - Yaws
KW - Molecular typing
KW - Multilocus analysis
KW - SS14-like strains
N2 - Treponema pallidum strains are closely related at the genome level but cause distinct diseases. Subspecies pallidum (TPA) is the causative agent of syphilis, subspecies pertenue (TPE) causes yaws while subspecies endemicum (TEN) causes bejel (endemic syphilis). Compared to the majority of treponemal genomic regions, several chromosomal loci were found to be more diverse. To assess genetic variability in diverse genomic positions, we have selected (based on published genomic data) and sequenced five variable loci, TP0304, TP0346, TPO488, TP0515 and TP0558, in 19 reference Treponema pallidum strains including all T. pallidum subspecies (TPA, TPE and TEN). Results of this multilocus analysis divided syphilitic isolates into two groups: SS14-like and Nichols-like. The SS14-like group is comprised of SS14, Grady, Mexico A and Philadelphia 1 strains. The Nichols-like group consisted of strains Nichols, Bal 73-1, DAL-1, MN-3, Philadelphia 2, Haiti B and Madras. The TP0558 locus was selected for further studies because it clearly distinguished between the SS14- and Nichols-like groups and because the phylogenetic tree derived from the TP0558 locus showed the same clustering pattern as the tree constructed from whole genome sequences. In addition, TP0558 was shown as the only tested locus that evolved under negative selection within TPA strains. Sequencing of a short fragment (573 bp) of the TP0558 locus in a set of 25 clinical isolates from 22 patients collected in the Czech Republic during 2012-2013 revealed that clinical isolates follow the SS14- and Nichols-like distribution. (C) 2014 Elsevier GmbH. All rights reserved.
ER -
NECHVÁTAL, Lukáš, Helena PĚTROŠOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Lenka MIKALOVÁ, Radim STRNADEL, Ivana KUKLOVÁ, Martina KOJANOVÁ, Miluše KREIDLOVÁ, Daniela VAŇOUSOVÁ, Přemysl PROCHÁZKA, Hana ZÁKOUCKÁ, Alena KRCHŇÁKOVÁ and David ŠMAJS. Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains. \textit{International Journal of Medical Microbiology}. JENA: Urban \&{} Fischer Verlag, 2014, vol.~304, 5-6, p.~645-653. ISSN~1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2014.04.007.
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