Syphilis-causing strains belong to separate SS14-like or
Nichols-like groups as defined by multilocus analysis of 19
Treponema pallidum strains

J 2014

Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains

NECHVÁTAL, Lukáš, Helena PĚTROŠOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Lenka MIKALOVÁ et. al.

Basic information

Original name

Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains

Authors

NECHVÁTAL, Lukáš (203 Czech Republic, belonging to the institution), Helena PĚTROŠOVÁ (203 Czech Republic, belonging to the institution), Linda GRILLOVÁ (203 Czech Republic, belonging to the institution), Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Lenka MIKALOVÁ (203 Czech Republic, belonging to the institution), Radim STRNADEL (203 Czech Republic), Ivana KUKLOVÁ (203 Czech Republic), Martina KOJANOVÁ (203 Czech Republic), Miluše KREIDLOVÁ (203 Czech Republic), Daniela VAŇOUSOVÁ (203 Czech Republic), Přemysl PROCHÁZKA (203 Czech Republic), Hana ZÁKOUCKÁ (203 Czech Republic), Alena KRCHŇÁKOVÁ (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Medical Microbiology, JENA, Urban & Fischer Verlag, 2014, 1438-4221

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.614

RIV identification code

RIV/00216224:14110/14:00073673

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.ijmm.2014.04.007

UT WoS

000339775600016

Keywords in English

Treponema pallidum; Syphilis; Yaws; Molecular typing; Multilocus analysis; SS14-like strains

Abstract

V originále

Treponema pallidum strains are closely related at the genome level but cause distinct diseases. Subspecies pallidum (TPA) is the causative agent of syphilis, subspecies pertenue (TPE) causes yaws while subspecies endemicum (TEN) causes bejel (endemic syphilis). Compared to the majority of treponemal genomic regions, several chromosomal loci were found to be more diverse. To assess genetic variability in diverse genomic positions, we have selected (based on published genomic data) and sequenced five variable loci, TP0304, TP0346, TPO488, TP0515 and TP0558, in 19 reference Treponema pallidum strains including all T. pallidum subspecies (TPA, TPE and TEN). Results of this multilocus analysis divided syphilitic isolates into two groups: SS14-like and Nichols-like. The SS14-like group is comprised of SS14, Grady, Mexico A and Philadelphia 1 strains. The Nichols-like group consisted of strains Nichols, Bal 73-1, DAL-1, MN-3, Philadelphia 2, Haiti B and Madras. The TP0558 locus was selected for further studies because it clearly distinguished between the SS14- and Nichols-like groups and because the phylogenetic tree derived from the TP0558 locus showed the same clustering pattern as the tree constructed from whole genome sequences. In addition, TP0558 was shown as the only tested locus that evolved under negative selection within TPA strains. Sequencing of a short fragment (573 bp) of the TP0558 locus in a set of 25 clinical isolates from 22 patients collected in the Czech Republic during 2012-2013 revealed that clinical isolates follow the SS14- and Nichols-like distribution. (C) 2014 Elsevier GmbH. All rights reserved.

Links

GAP302/12/0574, research and development project

Name: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Czech Science Foundation

NT11159, research and development project

Name: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministry of Health of the CR

Citovat

NECHVÁTAL, Lukáš, Helena PĚTROŠOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Lenka MIKALOVÁ, Radim STRNADEL, Ivana KUKLOVÁ, Martina KOJANOVÁ, Miluše KREIDLOVÁ, Daniela VAŇOUSOVÁ, Přemysl PROCHÁZKA, Hana ZÁKOUCKÁ, Alena KRCHŇÁKOVÁ and David ŠMAJS. Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains. International Journal of Medical Microbiology. JENA: Urban & Fischer Verlag, 2014, vol. 304, 5-6, p. 645-653. ISSN 1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2014.04.007.

@article{1185430,
   author = {Nechvátal, Lukáš and Pětrošová, Helena and Grillová, Linda and Pospíšilová, Petra and Mikalová, Lenka and Strnadel, Radim and Kuklová, Ivana and Kojanová, Martina and Kreidlová, Miluše and Vaňousová, Daniela and Procházka, Přemysl and Zákoucká, Hana and Krchňáková, Alena and Šmajs, David},
   article_location = {JENA},
   article_number = {5-6},
   doi = {http://dx.doi.org/10.1016/j.ijmm.2014.04.007},
   keywords = {Treponema pallidum; Syphilis; Yaws; Molecular typing; Multilocus analysis; SS14-like strains},
   language = {eng},
   issn = {1438-4221},
   journal = {International Journal of Medical Microbiology},
   title = {Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains},
   volume = {304},
   year = {2014}
}

TY  - JOUR
ID  - 1185430
AU  - Nechvátal, Lukáš - Pětrošová, Helena - Grillová, Linda - Pospíšilová, Petra - Mikalová, Lenka - Strnadel, Radim - Kuklová, Ivana - Kojanová, Martina - Kreidlová, Miluše - Vaňousová, Daniela - Procházka, Přemysl - Zákoucká, Hana - Krchňáková, Alena - Šmajs, David
PY  - 2014
TI  - Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains
JF  - International Journal of Medical Microbiology
VL  - 304
IS  - 5-6
SP  - 645-653
EP  - 645-653
PB  - Urban & Fischer Verlag
SN  - 14384221
KW  - Treponema pallidum
KW  - Syphilis
KW  - Yaws
KW  - Molecular typing
KW  - Multilocus analysis
KW  - SS14-like strains
N2  - Treponema pallidum strains are closely related at the genome level but cause distinct diseases. Subspecies pallidum (TPA) is the causative agent of syphilis, subspecies pertenue (TPE) causes yaws while subspecies endemicum (TEN) causes bejel (endemic syphilis). Compared to the majority of treponemal genomic regions, several chromosomal loci were found to be more diverse. To assess genetic variability in diverse genomic positions, we have selected (based on published genomic data) and sequenced five variable loci, TP0304, TP0346, TPO488, TP0515 and TP0558, in 19 reference Treponema pallidum strains including all T. pallidum subspecies (TPA, TPE and TEN). Results of this multilocus analysis divided syphilitic isolates into two groups: SS14-like and Nichols-like. The SS14-like group is comprised of SS14, Grady, Mexico A and Philadelphia 1 strains. The Nichols-like group consisted of strains Nichols, Bal 73-1, DAL-1, MN-3, Philadelphia 2, Haiti B and Madras. The TP0558 locus was selected for further studies because it clearly distinguished between the SS14- and Nichols-like groups and because the phylogenetic tree derived from the TP0558 locus showed the same clustering pattern as the tree constructed from whole genome sequences. In addition, TP0558 was shown as the only tested locus that evolved under negative selection within TPA strains. Sequencing of a short fragment (573 bp) of the TP0558 locus in a set of 25 clinical isolates from 22 patients collected in the Czech Republic during 2012-2013 revealed that clinical isolates follow the SS14- and Nichols-like distribution. (C) 2014 Elsevier GmbH. All rights reserved.
ER  -

NECHVÁTAL, Lukáš, Helena PĚTROŠOVÁ, Linda GRILLOVÁ, Petra POSPÍŠILOVÁ, Lenka MIKALOVÁ, Radim STRNADEL, Ivana KUKLOVÁ, Martina KOJANOVÁ, Miluše KREIDLOVÁ, Daniela VAŇOUSOVÁ, Přemysl PROCHÁZKA, Hana ZÁKOUCKÁ, Alena KRCHŇÁKOVÁ and David ŠMAJS. Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains. \textit{International Journal of Medical Microbiology}. JENA: Urban \&{} Fischer Verlag, 2014, vol.~304, 5-6, p.~645-653. ISSN~1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2014.04.007.

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