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@article{1186309, author = {Ogrocká, Anna and Polanská, Pavla and Majerová, Eva and Janeba, Zlatko and Fajkus, Jiří and Fojtová, Miloslava}, article_location = {OXFORD}, article_number = {5}, doi = {http://dx.doi.org/10.1093/nar/gkt1285}, keywords = {DNA METHYLATION; CYTOSINE METHYLATION; MAMMALIAN TELOMERES; EPIGENETIC INHERITANCE; CHROMOSOME ENDS; BINDING PROTEIN; NONCODING RNA; TOBACCO CELLS; GENOME; LENGTH}, language = {eng}, issn = {0305-1048}, journal = {Nucleic Acids Research}, title = {Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants}, url = {http://nar.oxfordjournals.org/content/42/5/2919.long}, volume = {42}, year = {2014} }
TY - JOUR ID - 1186309 AU - Ogrocká, Anna - Polanská, Pavla - Majerová, Eva - Janeba, Zlatko - Fajkus, Jiří - Fojtová, Miloslava PY - 2014 TI - Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants JF - Nucleic Acids Research VL - 42 IS - 5 SP - 2919-2931 EP - 2919-2931 PB - Oxford University Press SN - 03051048 KW - DNA METHYLATION KW - CYTOSINE METHYLATION KW - MAMMALIAN TELOMERES KW - EPIGENETIC INHERITANCE KW - CHROMOSOME ENDS KW - BINDING PROTEIN KW - NONCODING RNA KW - TOBACCO CELLS KW - GENOME KW - LENGTH UR - http://nar.oxfordjournals.org/content/42/5/2919.long L2 - http://nar.oxfordjournals.org/content/42/5/2919.long N2 - Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are important for the maintenance of genomic stability. Telomeres were considered as typical heterochromatic regions, but in light of recent results, this view should be reconsidered. Asymmetrically located cytosines in plant telomeric DNA repeats may be substrates for a DNA methyltransferase enzyme and indeed, it was shown that these repeats are methylated. Here, we analyse the methylation of telomeric cytosines and the length of telomeres in Arabidopsis thaliana methylation mutants (met 1-3 and ddm 1-8), and in their wild-type siblings that were germinated in the presence of hypomethylation drugs. Our results show that cytosine methylation in telomeric repeats depends on the activity of MET1 and DDM1 enzymes. Significantly shortened telomeres occur in later generations of methylation mutants as well as in plants germinated in the presence of hypomethylation drugs, and this phenotype is stably transmitted to the next plant generation. A possible role of compromised in vivo telomerase action in the observed telomere shortening is hypothesized based on telomere analysis of hypomethylated telomerase knockout plants. Results are discussed in connection with previous data in this field obtained using different model systems. ER -
OGROCKÁ, Anna, Pavla POLANSKÁ, Eva MAJEROVÁ, Zlatko JANEBA, Jiří FAJKUS and Miloslava FOJTOVÁ. Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants. \textit{Nucleic Acids Research}. OXFORD: Oxford University Press, vol.~42, No~5, p.~2919-2931. ISSN~0305-1048. doi:10.1093/nar/gkt1285. 2014.
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