OGROCKÁ, Anna, Pavla POLANSKÁ, Eva MAJEROVÁ, Zlatko JANEBA, Jiří FAJKUS and Miloslava FOJTOVÁ. Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants. Nucleic Acids Research. OXFORD: Oxford University Press, vol. 42, No 5, p. 2919-2931. ISSN 0305-1048. doi:10.1093/nar/gkt1285. 2014.
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Basic information
Original name Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants
Authors OGROCKÁ, Anna (203 Czech Republic, belonging to the institution), Pavla POLANSKÁ (203 Czech Republic, belonging to the institution), Eva MAJEROVÁ (203 Czech Republic, belonging to the institution), Zlatko JANEBA (203 Czech Republic), Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution) and Miloslava FOJTOVÁ (203 Czech Republic, belonging to the institution).
Edition Nucleic Acids Research, OXFORD, Oxford University Press, 2014, 0305-1048.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 9.112
RIV identification code RIV/00216224:14740/14:00073694
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1093/nar/gkt1285
UT WoS 000333093600018
Keywords in English DNA METHYLATION; CYTOSINE METHYLATION; MAMMALIAN TELOMERES; EPIGENETIC INHERITANCE; CHROMOSOME ENDS; BINDING PROTEIN; NONCODING RNA; TOBACCO CELLS; GENOME; LENGTH
Tags kontrola MP, OA, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 31/10/2014 15:38.
Abstract
Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are important for the maintenance of genomic stability. Telomeres were considered as typical heterochromatic regions, but in light of recent results, this view should be reconsidered. Asymmetrically located cytosines in plant telomeric DNA repeats may be substrates for a DNA methyltransferase enzyme and indeed, it was shown that these repeats are methylated. Here, we analyse the methylation of telomeric cytosines and the length of telomeres in Arabidopsis thaliana methylation mutants (met 1-3 and ddm 1-8), and in their wild-type siblings that were germinated in the presence of hypomethylation drugs. Our results show that cytosine methylation in telomeric repeats depends on the activity of MET1 and DDM1 enzymes. Significantly shortened telomeres occur in later generations of methylation mutants as well as in plants germinated in the presence of hypomethylation drugs, and this phenotype is stably transmitted to the next plant generation. A possible role of compromised in vivo telomerase action in the observed telomere shortening is hypothesized based on telomere analysis of hypomethylated telomerase knockout plants. Results are discussed in connection with previous data in this field obtained using different model systems.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GAP501/11/0596, research and development projectName: Epigenetické mechanismy regulace rostlinných telomer (Acronym: epigenetika telomer)
Investor: Czech Science Foundation
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