| HAFIDH, Said, David POTĚŠIL, Jan FÍLA, Jana FECÍKOVÁ, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL and David HONYS. In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception. BIOCHEMICAL SOCIETY TRANSACTIONS. LONDON: PORTLAND PRESS LTD, 2014, vol. 42, April, p. 388-394. ISSN 0300-5127. Available from: https://dx.doi.org/10.1042/BST20130204. |
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@article{1186360,
author = {Hafidh, Said and Potěšil, David and Fíla, Jan and Fecíková, Jana and Čapková, Věra and Zdráhal, Zbyněk and Honys, David},
article_location = {LONDON},
article_number = {April},
doi = {http://dx.doi.org/10.1042/BST20130204},
keywords = {GPI-ANCHORED PROTEIN; PLANT SECRETOME; ARABIDOPSIS; GROWTH; GUIDANCE; GERMINATION; ATTRACTANT; COMPONENTS; MAIZE; CELL},
language = {eng},
issn = {0300-5127},
journal = {BIOCHEMICAL SOCIETY TRANSACTIONS},
title = {In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception},
url = {http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm},
volume = {42},
year = {2014}
}
TY - JOUR
ID - 1186360
AU - Hafidh, Said - Potěšil, David - Fíla, Jan - Fecíková, Jana - Čapková, Věra - Zdráhal, Zbyněk - Honys, David
PY - 2014
TI - In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception
JF - BIOCHEMICAL SOCIETY TRANSACTIONS
VL - 42
IS - April
SP - 388-394
EP - 388-394
PB - PORTLAND PRESS LTD
SN - 03005127
KW - GPI-ANCHORED PROTEIN
KW - PLANT SECRETOME
KW - ARABIDOPSIS
KW - GROWTH
KW - GUIDANCE
KW - GERMINATION
KW - ATTRACTANT
KW - COMPONENTS
KW - MAIZE
KW - CELL
UR - http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm
L2 - http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm
N2 - The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome.
ER -
HAFIDH, Said, David POTĚŠIL, Jan FÍLA, Jana FECÍKOVÁ, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL and David HONYS. In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception. \textit{BIOCHEMICAL SOCIETY TRANSACTIONS}. LONDON: PORTLAND PRESS LTD, 2014, vol.~42, April, p.~388-394. ISSN~0300-5127. Available from: https://dx.doi.org/10.1042/BST20130204.
|
