In search of ligands and receptors of the pollen tube: the
missing link in pollen tube perception

J 2014

In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception

HAFIDH, Said, David POTĚŠIL, Jan FÍLA, Jana FECÍKOVÁ, Věra ČAPKOVÁ et. al.

Basic information

Original name

In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception

Authors

HAFIDH, Said (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Jan FÍLA (203 Czech Republic), Jana FECÍKOVÁ (203 Czech Republic), Věra ČAPKOVÁ (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution) and David HONYS (203 Czech Republic)

Edition

BIOCHEMICAL SOCIETY TRANSACTIONS, LONDON, PORTLAND PRESS LTD, 2014, 0300-5127

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.194

RIV identification code

RIV/00216224:14740/14:00073698

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1042/BST20130204

UT WoS

000333658900028

Keywords in English

GPI-ANCHORED PROTEIN; PLANT SECRETOME; ARABIDOPSIS; GROWTH; GUIDANCE; GERMINATION; ATTRACTANT; COMPONENTS; MAIZE; CELL

Abstract

V originále

The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GAP501/11/1462, research and development project

Name: Funkční charakterizace unikátních zásobních ribonukleoproteinových částic v pylu

Investor: Czech Science Foundation

Citovat

HAFIDH, Said, David POTĚŠIL, Jan FÍLA, Jana FECÍKOVÁ, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL and David HONYS. In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception. BIOCHEMICAL SOCIETY TRANSACTIONS. LONDON: PORTLAND PRESS LTD, 2014, vol. 42, April, p. 388-394. ISSN 0300-5127. Available from: https://dx.doi.org/10.1042/BST20130204.

@article{1186360,
   author = {Hafidh, Said and Potěšil, David and Fíla, Jan and Fecíková, Jana and Čapková, Věra and Zdráhal, Zbyněk and Honys, David},
   article_location = {LONDON},
   article_number = {April},
   doi = {http://dx.doi.org/10.1042/BST20130204},
   keywords = {GPI-ANCHORED PROTEIN; PLANT SECRETOME; ARABIDOPSIS; GROWTH; GUIDANCE; GERMINATION; ATTRACTANT; COMPONENTS; MAIZE; CELL},
   language = {eng},
   issn = {0300-5127},
   journal = {BIOCHEMICAL SOCIETY TRANSACTIONS},
   title = {In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception},
   url = {http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm},
   volume = {42},
   year = {2014}
}

TY  - JOUR
ID  - 1186360
AU  - Hafidh, Said - Potěšil, David - Fíla, Jan - Fecíková, Jana - Čapková, Věra - Zdráhal, Zbyněk - Honys, David
PY  - 2014
TI  - In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception
JF  - BIOCHEMICAL SOCIETY TRANSACTIONS
VL  - 42
IS  - April
SP  - 388-394
EP  - 388-394
PB  - PORTLAND PRESS LTD
SN  - 03005127
KW  - GPI-ANCHORED PROTEIN
KW  - PLANT SECRETOME
KW  - ARABIDOPSIS
KW  - GROWTH
KW  - GUIDANCE
KW  - GERMINATION
KW  - ATTRACTANT
KW  - COMPONENTS
KW  - MAIZE
KW  - CELL
UR  - http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm
L2  - http://www.biochemsoctrans.org/bst/042/0388/bst0420388.htm
N2  - The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome.
ER  -

HAFIDH, Said, David POTĚŠIL, Jan FÍLA, Jana FECÍKOVÁ, Věra ČAPKOVÁ, Zbyněk ZDRÁHAL and David HONYS. In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception. \textit{BIOCHEMICAL SOCIETY TRANSACTIONS}. LONDON: PORTLAND PRESS LTD, 2014, vol.~42, April, p.~388-394. ISSN~0300-5127. Available from: https://dx.doi.org/10.1042/BST20130204.

Detailed Information on Publication Record