Lactobacillus apis sp. nov., from the stomach of honeybees
(Apis mellifera), having an in vitro inhibitory effect on the
causative agents of American and European foulbrood

J 2014

Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood

KILLER, Jiří, Soňa DUBNÁ, Ivo SEDLÁČEK and Pavel ŠVEC

Basic information

Original name

Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood

Authors

KILLER, Jiří (203 Czech Republic, guarantor), Soňa DUBNÁ (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution) and Pavel ŠVEC (203 Czech Republic, belonging to the institution)

Edition

International Journal of Systematic and Evolutionary Microbiology, Society for General Microbiology, 2014, 1466-5026

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.511

RIV identification code

RIV/00216224:14310/14:00075988

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1099/ijs.0.053033-0

UT WoS

000337930400025

Keywords in English

Lactobacillus apis sp. nov.; Apis mellifera; taxonomy

Abstract

V originále

A taxonomic study was performed on Gram-stain-positive, catalase-negative and regular rod-shaped bacterial strains R4BT and R4C, isolated from the stomachs of honeybees. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strains was within the genus Lactobacillus; the highest sequence similarity to R4BT was shown by Lactobacillus acidophilus BCRC 10695T. Lower sequence similarities were found to other obligately homofermentative lactobacilli. A PCR–DGGE method could detect the sequence of the 16S rRNA gene of strain R4BT at different developmental stages of honeybees occurring in two different locations in the Czech Republic. The distinctiveness of the strains from other lactobacilli was also confirmed by analysis of sequences of other phylogenetic markers applicable to the taxonomy of the genus Lactobacillus, ribotyping and rep-PCR analysis. The DNA G+C content of strain R4BT was 41.3% mol. The major polar lipids of strain R4BT were glycolipids, lipids and phospholipids. Phenotypic and phylogenetic characteristics also confirmed the independent status of the strains at the species level. Interestingly, strain R4BT was able to inhibit growth in vitro of Paenibacillus larvae subsp. larvae (causal agent of American foulbrood in honeybees) and Melissococcus plutonius (causal agent of European foulbrood). The name Lactobacillus apis sp. nov. is proposed for this novel taxon.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

Citovat

KILLER, Jiří, Soňa DUBNÁ, Ivo SEDLÁČEK and Pavel ŠVEC. Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood. International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology, 2014, vol. 64, January, p. 152-157. ISSN 1466-5026. Available from: https://dx.doi.org/10.1099/ijs.0.053033-0.

@article{1192601,
   author = {Killer, Jiří and Dubná, Soňa and Sedláček, Ivo and Švec, Pavel},
   article_number = {January},
   doi = {http://dx.doi.org/10.1099/ijs.0.053033-0},
   keywords = {Lactobacillus apis sp. nov.; Apis mellifera; taxonomy},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood},
   url = {http://ijs.sgmjournals.org/content/64/Pt_1/152.abstract?sid=bc11a653-d638-446b-b762-9ec0c3cdb393},
   volume = {64},
   year = {2014}
}

TY  - JOUR
ID  - 1192601
AU  - Killer, Jiří - Dubná, Soňa - Sedláček, Ivo - Švec, Pavel
PY  - 2014
TI  - Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 64
IS  - January
SP  - 152-157
EP  - 152-157
PB  - Society for General Microbiology
SN  - 14665026
KW  - Lactobacillus apis sp. nov.
KW  - Apis mellifera
KW  - taxonomy
UR  - http://ijs.sgmjournals.org/content/64/Pt_1/152.abstract?sid=bc11a653-d638-446b-b762-9ec0c3cdb393
N2  - A taxonomic study was performed on Gram-stain-positive, catalase-negative and regular rod-shaped bacterial strains R4BT and R4C, isolated from the stomachs of honeybees. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strains was within the genus Lactobacillus; the highest sequence similarity to R4BT was shown by Lactobacillus acidophilus BCRC 10695T. Lower sequence similarities were found to other obligately homofermentative lactobacilli. A PCR–DGGE method could detect the sequence of the 16S rRNA gene of strain R4BT at different developmental stages of honeybees occurring in two different locations in the Czech Republic. The distinctiveness of the strains from other lactobacilli was also confirmed by analysis of sequences of other phylogenetic markers applicable to the taxonomy of the genus Lactobacillus, ribotyping and rep-PCR analysis. The DNA G+C content of strain R4BT was 41.3% mol. The major polar lipids of strain R4BT were glycolipids, lipids and phospholipids. Phenotypic and phylogenetic characteristics also confirmed the independent status of the strains at the species level. Interestingly, strain R4BT was able to inhibit growth in vitro of Paenibacillus larvae subsp. larvae (causal agent of American foulbrood in honeybees) and Melissococcus plutonius (causal agent of European foulbrood). The name Lactobacillus apis sp. nov. is proposed for this novel taxon.
ER  -

KILLER, Jiří, Soňa DUBNÁ, Ivo SEDLÁČEK and Pavel ŠVEC. Lactobacillus apis sp. nov., from the stomach of honeybees (Apis mellifera), having an in vitro inhibitory effect on the causative agents of American and European foulbrood. \textit{International Journal of Systematic and Evolutionary Microbiology}. Society for General Microbiology, 2014, vol.~64, January, p.~152-157. ISSN~1466-5026. Available from: https://dx.doi.org/10.1099/ijs.0.053033-0.

Detailed Information on Publication Record