CIP, Ondrej, Zdenek BUCHTA, Adam LESUNDAK, Antonin RANDULA, Bretislav MIKEL, Josef LAZAR and Lenka VEVERKOVÁ. Laser-induced fluorescence spectroscopy in tissue local necrosis detection. Online. In Jansen, ED; Thomas, RJ; Wilmink, GJ; Ibey, BL. OPTICAL INTERACTIONS WITH TISSUE AND CELLS XXV; AND TERAHERTZ FOR BIOMEDICAL APPLICATIONS. San Francisco: SPIE, 2014, p. 1-6. ISBN 978-0-8194-9854-0. Available from: https://dx.doi.org/10.1117/12.2052637.
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Basic information
Original name Laser-induced fluorescence spectroscopy in tissue local necrosis detection
Authors CIP, Ondrej (203 Czech Republic, guarantor), Zdenek BUCHTA (203 Czech Republic), Adam LESUNDAK (203 Czech Republic), Antonin RANDULA (203 Czech Republic), Bretislav MIKEL (203 Czech Republic), Josef LAZAR (203 Czech Republic) and Lenka VEVERKOVÁ (203 Czech Republic, belonging to the institution).
Edition San Francisco, OPTICAL INTERACTIONS WITH TISSUE AND CELLS XXV; AND TERAHERTZ FOR BIOMEDICAL APPLICATIONS, p. 1-6, 6 pp. 2014.
Publisher SPIE
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10306 Optics
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
RIV identification code RIV/00216224:14110/14:00076000
Organization unit Faculty of Medicine
ISBN 978-0-8194-9854-0
ISSN 0277-786X
Doi http://dx.doi.org/10.1117/12.2052637
UT WoS 000337580600026
Keywords in English IndoCyanine Green; fluorescence spectroscopy; small intestine; surgery
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 14/4/2015 08:36.
Abstract
The recent effort leads to reliable imaging techniques which can help to a surgeon during operations. The fluorescence spectroscopy was selected as very useful online in vivo imaging method to organics and biological materials analysis. The presented work scopes to a laser induced fluorescence spectroscopy technique to detect tissue local necrosis in small intestine surgery. In first experiments, we tested tissue auto-fluorescence technique but a signal-to-noise ratio didn't express significant results. Then we applied a contrast dye - IndoCyanine Green (ICG) which absorbs and emits wavelengths in the near IR. We arranged the pilot experimental setup based on highly coherent extended cavity diode laser (ECDL) used for stimulating of some critical areas of the small intestine tissue with injected ICG dye. We demonstrated the distribution of the ICG exciter with the first file of shots of small intestine tissue of a rabbit that was captured by high sensitivity fluorescent cam
Links
EE2.4.31.0016, research and development projectName: MEDTECH - vzdělávací a výzkumná partnerská síť v medicíně, biomedicíně a přístrojové technice
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