CIP, Ondrej, Zdenek BUCHTA, Adam LESUNDAK, Antonin RANDULA, Bretislav MIKEL, Josef LAZAR and Lenka VEVERKOVÁ. Laser-induced fluorescence spectroscopy in tissue local necrosis detection. Online. In Jansen, ED; Thomas, RJ; Wilmink, GJ; Ibey, BL. OPTICAL INTERACTIONS WITH TISSUE AND CELLS XXV; AND TERAHERTZ FOR BIOMEDICAL APPLICATIONS. San Francisco: SPIE, 2014, p. 1-6. ISBN 978-0-8194-9854-0. Available from: https://dx.doi.org/10.1117/12.2052637. |
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@inproceedings{1192687, author = {Cip, Ondrej and Buchta, Zdenek and Lesundak, Adam and Randula, Antonin and Mikel, Bretislav and Lazar, Josef and Veverková, Lenka}, address = {San Francisco}, booktitle = {OPTICAL INTERACTIONS WITH TISSUE AND CELLS XXV; AND TERAHERTZ FOR BIOMEDICAL APPLICATIONS}, doi = {http://dx.doi.org/10.1117/12.2052637}, editor = {Jansen, ED; Thomas, RJ; Wilmink, GJ; Ibey, BL}, keywords = {IndoCyanine Green; fluorescence spectroscopy; small intestine; surgery}, howpublished = {elektronická verze "online"}, language = {eng}, location = {San Francisco}, isbn = {978-0-8194-9854-0}, pages = {1-6}, publisher = {SPIE}, title = {Laser-induced fluorescence spectroscopy in tissue local necrosis detection}, year = {2014} }
TY - JOUR ID - 1192687 AU - Cip, Ondrej - Buchta, Zdenek - Lesundak, Adam - Randula, Antonin - Mikel, Bretislav - Lazar, Josef - Veverková, Lenka PY - 2014 TI - Laser-induced fluorescence spectroscopy in tissue local necrosis detection PB - SPIE CY - San Francisco SN - 9780819498540 KW - IndoCyanine Green KW - fluorescence spectroscopy KW - small intestine KW - surgery N2 - The recent effort leads to reliable imaging techniques which can help to a surgeon during operations. The fluorescence spectroscopy was selected as very useful online in vivo imaging method to organics and biological materials analysis. The presented work scopes to a laser induced fluorescence spectroscopy technique to detect tissue local necrosis in small intestine surgery. In first experiments, we tested tissue auto-fluorescence technique but a signal-to-noise ratio didn't express significant results. Then we applied a contrast dye - IndoCyanine Green (ICG) which absorbs and emits wavelengths in the near IR. We arranged the pilot experimental setup based on highly coherent extended cavity diode laser (ECDL) used for stimulating of some critical areas of the small intestine tissue with injected ICG dye. We demonstrated the distribution of the ICG exciter with the first file of shots of small intestine tissue of a rabbit that was captured by high sensitivity fluorescent cam ER -
CIP, Ondrej, Zdenek BUCHTA, Adam LESUNDAK, Antonin RANDULA, Bretislav MIKEL, Josef LAZAR and Lenka VEVERKOVÁ. Laser-induced fluorescence spectroscopy in tissue local necrosis detection. Online. In Jansen, ED; Thomas, RJ; Wilmink, GJ; Ibey, BL. \textit{OPTICAL INTERACTIONS WITH TISSUE AND CELLS XXV; AND TERAHERTZ FOR BIOMEDICAL APPLICATIONS}. San Francisco: SPIE, 2014, p.~1-6. ISBN~978-0-8194-9854-0. Available from: https://dx.doi.org/10.1117/12.2052637.
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