J 2014

Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts

DENEV, Petko, Maria KRATCHANOVA, Milan ČÍŽ, Antonín LOJEK, Ondřej VAŠÍČEK et. al.

Basic information

Original name

Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts

Authors

DENEV, Petko (100 Bulgaria, guarantor), Maria KRATCHANOVA (100 Bulgaria), Milan ČÍŽ (203 Czech Republic), Antonín LOJEK (203 Czech Republic), Ondřej VAŠÍČEK (203 Czech Republic), Denitsa BLAZHEVA (100 Bulgaria), Plamena NEDELCHEVA (100 Bulgaria), Libor VOJTEK (203 Czech Republic, belonging to the institution) and Pavel HYRŠL (203 Czech Republic, belonging to the institution)

Edition

Acta Biochimica Polonica, Warsaw, Acta Biochimica Polonica, 2014, 0001-527X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.153

RIV identification code

RIV/00216224:14310/14:00076005

Organization unit

Faculty of Science

UT WoS

000338392800023

Keywords in English

herbs; polyphenols; antioxidant activity; antimicrobial activity; phagocytes; reactive oxygen species

Tags

Tags

International impact, Reviewed
Změněno: 21/10/2019 08:23, Mgr. Marie Šípková, DiS.

Abstract

V originále

The present study provides a comprehensive data on the antioxidant, antimicrobial and neutrophil-modulating activities of extracts from six medicinal plants — blackberry (Rubus fruticosus) leaves, chokeberry (Aronia melanocarpa) leaves, hawthorn (Crataegus monogyna) leaves, lady’s mantle (Alchemilla glabra) aerial parts, meadowsweet (Filipendula ulmaria) aerial parts and raspberry (Rubus idaeus) leaves. In order to analyze the antioxidant activity of the herbs, several methods (ORAC, TRAP, HORAC and inhibition of lipid peroxidation) were used. Blackberry leaves and meadowsweet extracts revealed the highest antioxidant activities via all methods. All extracts studied blocked almost completely the opsonized zymosan particle-activated ROS production by neutrophils from human whole blood. On the other hand, the effect of extracts on phorbol myristate acetate-activated ROS production was much milder and even nonsignificant in the case of chokeberry leaves. This latter result suggests that extracts (apart from their antioxidative activity) interfere with the signaling cascade of phagocyte activation upstream of the protein kinase C activation. The antimicrobial activity of the investigated extracts against 11 human pathogens was investigated using three different methods. Meadowsweet and blackberry leaves extracts had the highest antimicrobial effect and the lowest minimal inhibiting concentrations (MICs) against the microorganisms tested.