KUDOVÁ, Jana, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK, Lucia BINÓ, Hana KOLÁŘOVÁ, Dominika SÝKOROVÁ, Pavel KARAS, Jiří PACHERNÍK and Lukáš KUBALA. The role of HIF-1α in the regulation of cardiomyogenesis in vitro. In XXXIV Annual Meeting of the North American Section, Miami Beach, Florida, USA. 2014.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name The role of HIF-1α in the regulation of cardiomyogenesis in vitro
Authors KUDOVÁ, Jana, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK, Lucia BINÓ, Hana KOLÁŘOVÁ, Dominika SÝKOROVÁ, Pavel KARAS, Jiří PACHERNÍK and Lukáš KUBALA.
Edition XXXIV Annual Meeting of the North American Section, Miami Beach, Florida, USA, 2014.
Other information
Type of outcome Conference abstract
Confidentiality degree is not subject to a state or trade secret
Tags International impact, Reviewed
Changed by Changed by: Mgr. Ondřej Vašíček, Ph.D., učo 141774. Changed: 1/8/2014 14:52.
Abstract
A cardiac cell formation (cardiomyogenesis) is critically dependent on cell microenvironment. Oxygen availability is one of the most important factors which plays an essential role in cardiomyocytes differentiation. It is known that hypoxia (a low oxygen level) modulates the differentiation of murine embryonic stem cells to cardiomyocytes in vitro. Hypoxic conditions lead to the stabilization of hypoxia-inducible factor-1 alpha (HIF-1alpha), the central mediator of cellular transcription responses to oxygen tension. However, the role of HIF-1alpha in the regulation of cardiomyocyte differentiation is not well understood. With the aim of better understanding the role of HIF-1alpha within cardiomyocyte development and progenitor cell reprogramming, cardiomyogenesis in wild type and HIF-1alpha depleted murine embryonic stem cells was analyzed in vitro. The level of expression of individual cardiospecific genes was higher in the HIF-1alpha deficient cell line compared to wild type cells in the early phase of differentiation (on Day 5). In contrast, in the late phase of cardiac cell development (on Day 15 and later), these markers were lower in HIF-1alpha deficient cells. This suggests a higher state of maturity in HIF-1alpha deficient cells at the beginning of cardiomyogenesis differentiation. At the same time, the profile of cardiospecific markers suggests that a lower number of progenitor cells enter the differentiation process. In conclusion, HIF-1alpha is important for induction of the development of cardiomyogenic progenitors and has a significant effect on the maturation of early cardiomyocytes in in vitro culture.
PrintDisplayed: 27/8/2024 13:17