CHLEBOWICZ, Monika A., Ivana MAŠLAŇOVÁ, Lucie KUNTOVÁ, Hajo GRUNDMANN, Roman PANTŮČEK, Jiří DOŠKAŘ, Jan Maarten VAN DIJL and Girbe BUIST. The Staphylococcal Cassette Chromosome mec type V from Staphylococcus aureus ST398 is packaged into bacteriophage capsids. International Journal of Medical Microbiology. Elsevier GmbH., 2014, vol. 304, 5-6, p. 764-774. ISSN 1438-4221. doi:10.1016/j.ijmm.2014.05.010.
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Basic information
Original name The Staphylococcal Cassette Chromosome mec type V from Staphylococcus aureus ST398 is packaged into bacteriophage capsids
Authors CHLEBOWICZ, Monika A. (616 Poland), Ivana MAŠLAŇOVÁ (203 Czech Republic, guarantor, belonging to the institution), Lucie KUNTOVÁ (203 Czech Republic, belonging to the institution), Hajo GRUNDMANN (528 Netherlands), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution), Jan Maarten VAN DIJL (528 Netherlands) and Girbe BUIST (528 Netherlands).
Edition International Journal of Medical Microbiology, Elsevier GmbH. 2014, 1438-4221.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.614
RIV identification code RIV/00216224:14310/14:00073787
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.ijmm.2014.05.010
UT WoS 000339775600030
Keywords (in Czech) Staphylococcus aureus; ST398; SCCmec; transdukce; bakteriofág
Keywords in English Staphylococcus aureus; ST398; SCCmec; Transduction; Bacteriophage
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 11/4/2015 22:52.
Abstract
The Staphylococcal Cassette Chromosome mec (SCCmec) confers methicillin resistance to Staphylococcus aureus. While SCCmec is generally regarded as a mobile genetic element, the precise mechanisms by which large SCCmec elements are exchanged between staphylococci have remained enigmatic. In the present studies, we observed that the clinical methicillin-resistant S. aureus (MRSA) isolate UMCG-M4 with the sequence type 398 contains four prophages belonging to the serological groups A, B and Fa. Previous studies have shown that certain serological group B bacteriophages of S. aureus are capable of generalized transduction. We therefore assessed the transducing capabilities of the phages from strain UMCG-M4. The results show that some of these phages can indeed transduce plasmid pT181 to the recipient S. aureus strain RN4220. Therefore, we also investigated the possible involvement of these transducing phages in the transmission of the large SCCmec type V (5C2&5) element of S. aureus UMCG-M4. While no transduction of the complete SCCmec element was observed, we were able to demonstrate that purified phage particles did contain large parts of the SCCmec element of the donor strain, including the methicillin resistance gene mecA. This shows that staphylococcal phages can encapsulate the resistance determinant mecA of a large SCCmec type V (5C2&5) element, which may lead to its transfer to other staphylococci.
Links
GP13-05069P, research and development projectName: Horizontální přenos stafylokokové chromozomové kazety mec (SCCmec) prostřednictvím bakteriofágů napříč kmeny rodu Staphylococcus
Investor: Czech Science Foundation, Postdoctoral projects
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