The purpose of this study was to assess the flow state of aikido practitioners. Seventy-six Czech male and female aikido practitioners (age 32.5±9.2 years (mean±SD), range 18-56 years) participated in this study. The subjects were divided according to a ranking system into three groups: Beginners (up to third kyu), Intermediate (second and first kyu) and Advanced (dan holders). The Flow State Scale-2 was used to assess the experience of flow in aikido. Practical significance was found in flow state in different levels of aikido practitioners. Partial eta-squared coefficient was 0.04 in beginners, intermediate as well as intermediate and advanced aikido students. Significant differences were found in Unambiguous feedback and Sense of control dimensions of flow state. No differences were found between female and male aikido practitioners too.