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@article{1195718,
author = {Adamová, Lenka and Malinovská, Lenka and Wimmerová, Michaela},
article_location = {USA},
article_number = {10},
doi = {http://dx.doi.org/10.1002/jemt.22407},
keywords = {hemagglutination; protein-carbohydrate interactions; blood group antigens; lectin},
language = {eng},
issn = {1059-910X},
journal = {Microscopy research and technique},
title = {New sensitive detection method for lectin hemagglutination using microscopy.},
url = {http://dx.doi.org/10.1002/jemt.22407},
volume = {77},
year = {2014}
}
TY - JOUR
ID - 1195718
AU - Adamová, Lenka - Malinovská, Lenka - Wimmerová, Michaela
PY - 2014
TI - New sensitive detection method for lectin hemagglutination using microscopy.
JF - Microscopy research and technique
VL - 77
IS - 10
SP - 841-849
EP - 841-849
PB - Wiley-Blackwell
SN - 1059910X
KW - hemagglutination
KW - protein-carbohydrate interactions
KW - blood group antigens
KW - lectin
UR - http://dx.doi.org/10.1002/jemt.22407
L2 - http://dx.doi.org/10.1002/jemt.22407
N2 - The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope. Comparison with the standard hemagglutination assay using microtiter plates revealed that microscopic approach is faster and more robust and allows fast determination of lectin activities immediately in bacterial cytosols.
ER -
ADAMOVÁ, Lenka, Lenka MALINOVSKÁ and Michaela WIMMEROVÁ. New sensitive detection method for lectin hemagglutination using microscopy. \textit{Microscopy research and technique}. USA: Wiley-Blackwell, 2014, vol.~77, No~10, p.~841-849. ISSN~1059-910X. Available from: https://dx.doi.org/10.1002/jemt.22407.
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