Detailed Information on Publication Record
2014
New sensitive detection method for lectin hemagglutination using microscopy.
ADAMOVÁ, Lenka, Lenka MALINOVSKÁ and Michaela WIMMEROVÁBasic information
Original name
New sensitive detection method for lectin hemagglutination using microscopy.
Name in Czech
Nová citlivá metoda pro lektinovou hemaglutinaci pomocí mikroskopu
Authors
ADAMOVÁ, Lenka (203 Czech Republic, belonging to the institution), Lenka MALINOVSKÁ (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Microscopy research and technique, USA, Wiley-Blackwell, 2014, 1059-910X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.154
RIV identification code
RIV/00216224:14740/14:00073809
Organization unit
Central European Institute of Technology
UT WoS
000342207900012
Keywords (in Czech)
hemaglutinace; protein-sacharidové interakce; antigeny krevních skupin; lektin
Keywords in English
hemagglutination; protein-carbohydrate interactions; blood group antigens; lectin
Tags
Tags
International impact, Reviewed
Změněno: 28/5/2015 09:11, prof. RNDr. Michaela Wimmerová, Ph.D.
Abstract
V originále
The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope. Comparison with the standard hemagglutination assay using microtiter plates revealed that microscopic approach is faster and more robust and allows fast determination of lectin activities immediately in bacterial cytosols.
Links
| CZ.1.05/1.1.00/02.0068, interní kód MU |
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| ED1.1.00/02.0068, research and development project |
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| EE2.3.30.0009, research and development project |
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| GA13-25401S, research and development project |
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