Detection of Aerosolized Biological Agents Using the
Piezoelectric Immunosensor

J 2014

Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor

KOVÁŘ, David, Zdeněk FARKA and Petr SKLÁDAL

Basic information

Original name

Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor

Authors

KOVÁŘ, David (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2014, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.636

RIV identification code

RIV/00216224:14740/14:00076277

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/ac501623m

UT WoS

000341229200026

Keywords in English

bioaerosol; quartz crystal microbalance; immunosensor; E. coli K-12; bioaerosol chamber

Abstract

V originále

Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator in the chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 × 104 CFU·L–1 of air. The total time from sample collection to detection was 16 min. The system was fully automated and controlled remotely through a local network.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

KOVÁŘ, David, Zdeněk FARKA and Petr SKLÁDAL. Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2014, vol. 86, No 17, p. 8680-8686. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/ac501623m.

@article{1196466,
   author = {Kovář, David and Farka, Zdeněk and Skládal, Petr},
   article_location = {Washington, D.C., USA},
   article_number = {17},
   doi = {http://dx.doi.org/10.1021/ac501623m},
   keywords = {bioaerosol; quartz crystal microbalance; immunosensor; E. coli K-12; bioaerosol chamber},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor},
   url = {http://pubs.acs.org/doi/abs/10.1021/ac501623m},
   volume = {86},
   year = {2014}
}

TY  - JOUR
ID  - 1196466
AU  - Kovář, David - Farka, Zdeněk - Skládal, Petr
PY  - 2014
TI  - Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor
JF  - Analytical Chemistry
VL  - 86
IS  - 17
SP  - 8680-8686
EP  - 8680-8686
PB  - American Chemical Society
SN  - 00032700
KW  - bioaerosol
KW  - quartz crystal microbalance
KW  - immunosensor
KW  - E. coli K-12
KW  - bioaerosol chamber
UR  - http://pubs.acs.org/doi/abs/10.1021/ac501623m
L2  - http://pubs.acs.org/doi/abs/10.1021/ac501623m
N2  - Airborne microorganisms are a major cause of respiratory diseases. Detection of pathogenic bacteria in the form of bioaerosols is required not only in peacetime but also in the threat of biological attacks. The label-free and direct detection of aerosolized biological agents is presented here. A desktop bioaerosol chamber for safe work with aerosolized microbial cells was constructed, and its functionality was tested. The model organisms (Escherichia coli) were disseminated using an aerosol generator in the chamber filled with either common laboratory indoor air or sterile air. The particles from the generated aerosol were collected using the cyclone SASS 2300, suspended in buffer and then analyzed using the piezoelectric immunosensor modified with specific capture antibodies. The frequency shifts indicated presence of the model biological agent with limit of detection of 1.45 × 104 CFU·L–1 of air. The total time from sample collection to detection was 16 min. The system was fully automated and controlled remotely through a local network.
ER  -

KOVÁŘ, David, Zdeněk FARKA and Petr SKLÁDAL. Detection of Aerosolized Biological Agents Using the Piezoelectric Immunosensor. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2014, vol.~86, No~17, p.~8680-8686. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/ac501623m.

Detailed Information on Publication Record