An Effect of Cadmium and Lead Ions on Escherichia coli with the
Cloned Gene for Metallothionein (MT-3) Revealed by
Electrochemistry

J 2014

An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry

ADAM, Vojtěch, Dagmar CHUDOBOVÁ, Kateřina TMEJOVÁ, Kristýna ČÍHALOVÁ, Soňa KŘÍŽKOVÁ et. al.

Basic information

Original name

An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry

Authors

ADAM, Vojtěch (203 Czech Republic), Dagmar CHUDOBOVÁ (203 Czech Republic), Kateřina TMEJOVÁ (203 Czech Republic), Kristýna ČÍHALOVÁ (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic), Roman GURÁŇ (203 Czech Republic), Markéta KOMÍNKOVÁ (203 Czech Republic), Michal ŽŮREK (203 Czech Republic), Monika KREMPLOVÁ (203 Czech Republic), Ana Maria JIMENEZ JIMENEZ (203 Czech Republic), Marie KONEČNÁ (203 Czech Republic), David HYNEK (203 Czech Republic), Vladimír PEKAŘÍK (203 Czech Republic, guarantor, belonging to the institution) and René KIZEK (203 Czech Republic)

Edition

Electrochimica Acta, Oxford, Pergamon- Elsevier Science Ltd, 2014, 0013-4686

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.504

RIV identification code

RIV/00216224:14740/14:00076328

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.electacta.2014.06.091

UT WoS

000342528600003

Keywords in English

Escherichia coli; Metallothionein; Voltammetry; Cloning; Metal Ions

Abstract

V originále

This study was focused on the application of electrochemical methods for studying of bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after the application of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 mikroM). Bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like model organisms for studying of metals influence to metallothionein expression. Metallothionein was isolated using fast protein liquid chromatography and quantified by electrochemical methods. The occurrence of metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15 (MT dimer) and 22 kDa (MT trimer). The changes in electrochemical records due to the interactions of metallothioneins (MT-3 and MT-2A) with cadmium and lead ions showed decline of Cat2 signal of MT with the increasing interaction time because of metal ions binding to cysteines. Electrochemical determination also revealed that Cd(II) remains in E. coli cells in the higher amount than Pb (II). Opposite situation was found at E. coli–MT-3 strain. The antimicrobial effect of cadmium ions was determined by IC50 and was statistically calculated as 39.2 and 95.5 mikroM for E. coli without cloned MT-3 and E. coli carrying MT-3 gene, respectively. High provided concentration IC50 in strains after lead ions application (352.5 mikroM for E. coli without cloning and 207.0 mikroM for E. coli carrying cloned MT-3 gene) indicates lower toxicity of lead ions on bacterial strains compared to the cadmium ions.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

ADAM, Vojtěch, Dagmar CHUDOBOVÁ, Kateřina TMEJOVÁ, Kristýna ČÍHALOVÁ, Soňa KŘÍŽKOVÁ, Roman GURÁŇ, Markéta KOMÍNKOVÁ, Michal ŽŮREK, Monika KREMPLOVÁ, Ana Maria JIMENEZ JIMENEZ, Marie KONEČNÁ, David HYNEK, Vladimír PEKAŘÍK and René KIZEK. An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry. Electrochimica Acta. Oxford: Pergamon- Elsevier Science Ltd, 2014, vol. 140, September, p. 11-19. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2014.06.091.

@article{1196637,
   author = {Adam, Vojtěch and Chudobová, Dagmar and Tmejová, Kateřina and Číhalová, Kristýna and Křížková, Soňa and Guráň, Roman and Komínková, Markéta and Žůrek, Michal and Kremplová, Monika and Jimenez Jimenez, Ana Maria and Konečná, Marie and Hynek, David and Pekařík, Vladimír and Kizek, René},
   article_location = {Oxford},
   article_number = {September},
   doi = {http://dx.doi.org/10.1016/j.electacta.2014.06.091},
   keywords = {Escherichia coli; Metallothionein; Voltammetry; Cloning; Metal Ions},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry},
   url = {http://www.sciencedirect.com/science/article/pii/S0013468614012730},
   volume = {140},
   year = {2014}
}

TY  - JOUR
ID  - 1196637
AU  - Adam, Vojtěch - Chudobová, Dagmar - Tmejová, Kateřina - Číhalová, Kristýna - Křížková, Soňa - Guráň, Roman - Komínková, Markéta - Žůrek, Michal - Kremplová, Monika - Jimenez Jimenez, Ana Maria - Konečná, Marie - Hynek, David - Pekařík, Vladimír - Kizek, René
PY  - 2014
TI  - An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry
JF  - Electrochimica Acta
VL  - 140
IS  - September
SP  - 11-19
EP  - 11-19
PB  - Pergamon- Elsevier Science Ltd
SN  - 00134686
KW  - Escherichia coli
KW  - Metallothionein
KW  - Voltammetry
KW  - Cloning
KW  - Metal Ions
UR  - http://www.sciencedirect.com/science/article/pii/S0013468614012730
L2  - http://www.sciencedirect.com/science/article/pii/S0013468614012730
N2  - This study was focused on the application of electrochemical methods for studying of bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after the application of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 mikroM). Bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like model organisms for studying of metals influence to metallothionein expression. Metallothionein was isolated using fast protein liquid chromatography and quantified by electrochemical methods. The occurrence of metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15 (MT dimer) and 22 kDa (MT trimer). The changes in electrochemical records due to the interactions of metallothioneins (MT-3 and MT-2A) with cadmium and lead ions showed decline of Cat2 signal of MT with the increasing interaction time because of metal ions binding to cysteines. Electrochemical determination also revealed that Cd(II) remains in E. coli cells in the higher amount than Pb (II). Opposite situation was found at E. coli–MT-3 strain. The antimicrobial effect of cadmium ions was determined by IC50 and was statistically calculated as 39.2 and 95.5 mikroM for E. coli without cloned MT-3 and E. coli carrying MT-3 gene, respectively. High provided concentration IC50 in strains after lead ions application (352.5 mikroM for E. coli without cloning and 207.0 mikroM for E. coli carrying cloned MT-3 gene) indicates lower toxicity of lead ions on bacterial strains compared to the cadmium ions.
ER  -

ADAM, Vojtěch, Dagmar CHUDOBOVÁ, Kateřina TMEJOVÁ, Kristýna ČÍHALOVÁ, Soňa KŘÍŽKOVÁ, Roman GURÁŇ, Markéta KOMÍNKOVÁ, Michal ŽŮREK, Monika KREMPLOVÁ, Ana Maria JIMENEZ JIMENEZ, Marie KONEČNÁ, David HYNEK, Vladimír PEKAŘÍK and René KIZEK. An Effect of Cadmium and Lead Ions on Escherichia coli with the Cloned Gene for Metallothionein (MT-3) Revealed by Electrochemistry. \textit{Electrochimica Acta}. Oxford: Pergamon- Elsevier Science Ltd, 2014, vol.~140, September, p.~11-19. ISSN~0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2014.06.091.

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