The protein composition of excretory-secretory products of three Trichinella species

ŠRÁMOVÁ, Eliška, Jana ILGOVÁ, Lucie ŠKORPÍKOVÁ, Břetislav KOUDELA, David POTĚŠIL, Zbyněk ZDRÁHAL, Petr HORÁK, Milan GELNAR and Martin KAŠNÝ. The protein composition of excretory-secretory products of three Trichinella species. In 13th ICOPA. 2014.

Basic information

• Original name: The protein composition of excretory-secretory products of three Trichinella species
• Authors: ŠRÁMOVÁ, Eliška (203 Czech Republic, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Lucie ŠKORPÍKOVÁ (203 Czech Republic, belonging to the institution), Břetislav KOUDELA (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Petr HORÁK (203 Czech Republic), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: 13th ICOPA, 2014.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Mexico
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/14:00076505
• Organization unit: Faculty of Science
• Keywords in English: Excretory-secretory products; trichinella; mass spectrometry

Abstract

The nematodes of the genus Trichinella are intracellular parasites of skeletal muscle cells. These organisms are able to parasitize carnivorous and omnivorous vertebrates, including man. They are causative agents of trichinellosis, an important worldwide zoonotic disease. Twelve Trichinella taxa are divided into two clades characterized by the presence or absence of an intramuscular collagen capsule. In the Czech Republic we recognize three trichinella species - Trichinella spiralis, T. britovi (mainly in carnivores and wild boars) and T. pseudospiralis (mainly in predatory birds). While T. spiralis and T. britovi induce the formation of a collagen capsule surrounding the parasite, T. pseudospiralis survives in a non-encapsulated form. Investigating the functions of various bioactive molecules produced by parasites expands our understanding to the principles of host-parasite interaction. We used High-Performance Liquid Chromatography (UltiMate® 3000 RSLCnano LC) in combination with Mass Spectrometry (Orbitrap-Elite MS system) and transcriptomic analysis (Illumina MiSeq) to reveal and compare the spectrum of molecules in excretory-secretory products and all transcripts of L1 larvae of these model organisms. Several of important functional protein compounds were observed and their key role in host-parasite cross-talking was estimated. 364 proteins in ES products of Trichinella spp. L1 larvae were identified, e.g. antigens - 53 kDa protein, 43 kDa glycoprotein or biologically active proteins – cysteine peptidases and their inhibitors. Some of these molecules were prepared in recombinant form.