Capacity of Antarctic lichen Xanthoria elegans to synthetize
photoprotective secondary compounds is affected by supplemetary
UV-B doses

a 2014

Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses

BALARINOVÁ, Kateřina, Jana HAZDROVÁ and Miloš BARTÁK

Basic information

Original name

Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses

Name in Czech

Kapacita syntézy fotoochranných sekundátních metabolitů je u lišejníku Xantoria elegans ovlivněna dávlou UV-B záření

Authors

BALARINOVÁ, Kateřina (203 Czech Republic, guarantor, belonging to the institution), Jana HAZDROVÁ (203 Czech Republic, belonging to the institution) and Miloš BARTÁK (203 Czech Republic, belonging to the institution)

Edition

2014

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Downloadable Book of Abstracts

RIV identification code

RIV/00216224:14310/14:00076586

Organization unit

Faculty of Science

ISBN

978-80-7394-463-6

Keywords (in Czech)

UV-B absorbující látky; fotoinhibice; ostrov Jamese Rosse; fluorescence chlorofylu; spektroskopie

Keywords in English

UV-B screens; photoinhibition; James Ross Island;chlorophyll fluorescence; spectroscopy

Změněno: 25/9/2014 10:38, prof. Ing. Miloš Barták, CSc.

Abstract

ORIG CZ

V originále

Lichen thalli of X. elegans were collected at James Ross Island (Antarctica). They were dried under natural field conditions and then transported to the laboratory in Brno, Czech Republic. Before experiments, the thalli were re-wetted for 48 h by demineralized water and wet cellulose sheet located beneath the thalli. During re-wetting, the thalli were exposed to a dim light (20 micromol m-2 s-1 of PAR) at 5oC. Duration of exposition to free supplemental UV-B was 7 days. Three different intensities of UV-B radiaton were used (0.9 W.m-2, 1.4 W.m-2 and 3.0 W.m-2, in the second cycle 0.9 W.m-2, 1.15 W.m-2, 1.4 W.m-2). Samples for further analysis were taken during the exposition after 5, 10, 24, 48 and 168 h. During the exposition chlorophyll fluorescence parameters were measured using an approach of Kautsky kinetics supplemented with quenching analysis. Time courses of the following parameters were measured: Fv/Fm, Yield PSII, non-photochemical quenching (NPQ, qN) during exposition: 5, 10, 24, 48, and 168 h. After the exposition, UV-B absorbing compounds, pigment contents and concentration of glutathione were measured. UV-B induced synthesis of photoprotective compounds was measured using spectral absorption curves of extracts made in ethanol extracts. Amount of UV-B absorbing compounds was then evaluated according to the analysis of spectral absorbance curves. Chlorophyll and carotenoid contents were measured by a spectrophotometre (Specord, Analytik Jena). Glutathione was analysed using a method of thiol groups labeling.

In Czech

Prosím, přečtěte si abstrakt v angličtině.

Links

LM2010009, research and development project

Name: Projekt CzechPolar - České polární stanice: Stavba a operační náklady

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BALARINOVÁ, Kateřina, Jana HAZDROVÁ and Miloš BARTÁK. Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses. 2014. ISBN 978-80-7394-463-6.

@proceedings{1199186,
   author = {Balarinová, Kateřina and Hazdrová, Jana and Barták, Miloš},
   keywords = {UV-B screens; photoinhibition; James Ross Island;chlorophyll fluorescence; spectroscopy},
   language = {eng},
   isbn = {978-80-7394-463-6},
   title = {Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses},
   url = {http://polar.prf.jcu.cz/conference_MATERIALS.htm},
   year = {2014}
}

TY  - CONF
ID  - 1199186
AU  - Balarinová, Kateřina - Hazdrová, Jana - Barták, Miloš
PY  - 2014
TI  - Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses
SN  - 9788073944636
KW  - UV-B screens
KW  - photoinhibition
KW  - James Ross Island;chlorophyll fluorescence
KW  - spectroscopy
UR  - http://polar.prf.jcu.cz/conference_MATERIALS.htm
L2  - http://polar.prf.jcu.cz/conference_MATERIALS.htm
N2  - Lichen thalli of X. elegans were collected at James Ross Island (Antarctica). They were dried under natural field conditions and then transported to the laboratory in Brno, Czech Republic. Before experiments, the thalli were re-wetted for 48 h by demineralized water and wet cellulose sheet located beneath the thalli. During re-wetting, the thalli were exposed to a dim light (20 micromol m-2 s-1 of PAR) at 5oC. Duration of exposition to free supplemental UV-B was 7 days. Three different intensities of UV-B radiaton were used (0.9 W.m-2, 1.4 W.m-2 and 3.0 W.m-2, in the second cycle 0.9 W.m-2, 1.15 W.m-2, 1.4 W.m-2). Samples for further analysis were taken during the exposition after 5, 10, 24, 48 and 168 h. During the exposition chlorophyll fluorescence parameters were measured using an approach of Kautsky kinetics supplemented with quenching analysis. Time courses of the following parameters were measured: Fv/Fm, Yield PSII, non-photochemical quenching (NPQ, qN) during exposition: 5, 10, 24, 48, and 168 h. After the exposition, UV-B absorbing compounds, pigment contents and concentration of glutathione were measured. UV-B induced synthesis of photoprotective compounds was measured using spectral absorption curves of extracts made in ethanol extracts. Amount of UV-B absorbing compounds was then evaluated according to the analysis of spectral absorbance curves. Chlorophyll and carotenoid contents were measured by a spectrophotometre (Specord, Analytik Jena). Glutathione was analysed using a method of thiol groups labeling.
ER  -

BALARINOVÁ, Kateřina, Jana HAZDROVÁ and Miloš BARTÁK. \textit{Capacity of Antarctic lichen Xanthoria elegans to synthetize photoprotective secondary compounds is affected by supplemetary UV-B doses}. 2014. ISBN~978-80-7394-463-6.

Detailed Information on Publication Record