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@proceedings{1199198, author = {Hájek, Josef and Váczi, Peter and Hazdrová, Jana and Barták, Miloš}, booktitle = {Polar Ecology Conference, Ceske Budejovice, p. 56-57.}, keywords = {cryoresistance; chlorophyll fluorescence; thallus; lichen photobiont; critical temperature}, language = {eng}, isbn = {978-80-7394-463-6}, title = {Simultaneous measurements of PSII activity and thallus temperature during linear cooling. A method to assess interspecific differences in photosynthetic processes in lichens from polar regions.}, url = {http://polar.prf.jcu.cz/data/PEC2014_abstract_book_final.pdf}, year = {2014} }
TY - CONF ID - 1199198 AU - Hájek, Josef - Váczi, Peter - Hazdrová, Jana - Barták, Miloš PY - 2014 TI - Simultaneous measurements of PSII activity and thallus temperature during linear cooling. A method to assess interspecific differences in photosynthetic processes in lichens from polar regions. SN - 9788073944636 KW - cryoresistance KW - chlorophyll fluorescence KW - thallus KW - lichen photobiont KW - critical temperature UR - http://polar.prf.jcu.cz/data/PEC2014_abstract_book_final.pdf N2 - A comparison of cryoresistance of isolated lichen photobiont and polar lichens is presented. Several species were chosen for this experiment: (1) algal cultures of Trebouxia glomerata, Trebouxia sp. (2) thalli of Usnea antarctica, Usnea aurantiaco-atra, Usnea longissima, Umbilicaria decussata, Umbilicaria cylindrica. All the samples were exposed to temperatures linearly decreasing from +20 °C to -40 °C under controlled conditions. During the experiment, sample temperature and two parameters of chlorophyll fluorescence – (1) potential (FV/FM) and (2) effective quantum yield of photochemical processes in photosystem II (YieldPSII) – were measured simultaneously. Cooling was provided by a KRYO 560-16 (PLANER, United Kingdom) device. For majority of samples, two cooling rates were used: 6 °C min-1 (fast) and 2 °C min-1 (slow) were used. The chlorophyll fluorescence was measured by a PC-linked PAM 2000 fluorometer (H. Walz, Germany). Measurements of YieldPSII were made every 30 s. For all samples, the response of FV/FM and YieldPSII to declining temperature showed an „S curve“ with critical temperatures, indicating high level of cryoresistance of the samples. In an individual sample, critical temperature for FV/FM was typically lower than for YieldPSII. ER -
HÁJEK, Josef, Peter VÁCZI, Jana HAZDROVÁ and Miloš BARTÁK. Simultaneous measurements of PSII activity and thallus temperature during linear cooling. A method to assess interspecific differences in photosynthetic processes in lichens from polar regions. In \textit{Polar Ecology Conference, Ceske Budejovice, p. 56-57.}. 2014. ISBN~978-80-7394-463-6.
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