Detailed Information on Publication Record
2014
Dual effect of ethanol on inward rectifier potassium current Ik1 in rat ventricular myocytes
BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Michal PÁSEK, Milena ŠIMURDOVÁ, Jiří ŠIMURDA et. al.Basic information
Original name
Dual effect of ethanol on inward rectifier potassium current Ik1 in rat ventricular myocytes
Authors
BÉBAROVÁ, Markéta (203 Czech Republic, guarantor, belonging to the institution), Peter MATEJOVIČ (703 Slovakia, belonging to the institution), Michal PÁSEK (203 Czech Republic, belonging to the institution), Milena ŠIMURDOVÁ (203 Czech Republic, belonging to the institution) and Jiří ŠIMURDA (203 Czech Republic, belonging to the institution)
Edition
Journal of Physiology and Pharmacology, Kraków, Polish Physiological Society, 2014, 0867-5910
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30105 Physiology
Country of publisher
Poland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.386
RIV identification code
RIV/00216224:14110/14:00080144
Organization unit
Faculty of Medicine
Keywords in English
Arrhythmia; Dual effect; Ethanol; Inward rectifier; Rat ventricular action potential model; Rat ventricular myocytes
Tags
Tags
International impact, Reviewed
Změněno: 24/4/2015 13:43, Soňa Böhmová
Abstract
V originále
Alcohol consumption may result in electrocardiographic changes and arrhythmias. Important role of modifications of the inward rectifier potassium current IK1 in arrhythmogenesis is well established. Considering lack of relevant data, we aimed at studying the effect of 0.2-200 mM ethanol on IK1 in enzymatically isolated rat right ventricular myocytes using the whole cell patch-clamp technique at 23±1°C. Ethanol reversibly affected IK1 in a dual way. At a very low concentration of 0.8 mM (~0.004%), ethanol significantly decreased IK1 by 6.9±2.7%. However, at concentrations of ethanol >/- 20 mM (~0.09%), IK1 was conversely significantly increased (by 16.6±4.0% at 20 mM and 24.5±2.4% at 80 mM). The steady-state IK1 increase was regularly preceded by its transient decrease at the beginning of ethanol application. Under 2 and 8 mM ethanol, IK1 was decreased at the steady-state in some cells but increased in others. Both effects were voltage-independent. In agreement with the observed effects of ethanol on IK1, a transient action potential (AP) prolongation followed by its final shortening were observed after the application of ethanol in a low concentration of 8 mM (~0.04%). Under the effect of 0.8 mM ethanol, only AP prolongation was apparent which agreed well with the above described IK1 decrease. Other AP characteristics remained unaltered in both concentrations. These observations corresponded with the results of mathematical simulations in a model of the rat ventricular myocyte. To summarize, changes of the cardiac IK1 under ethanol at concentrations relevant to the current alcohol consumption were first demonstrated in ventricular myocytes in this study. The observed dual ethanol effect suggests at least two underlying mechanisms that remain to be clarified. The ethanol-induced IK1 changes might contribute to the reported alterations of cardiac electrophysiology related to alcohol consumption.
Links
| NT14301, research and development project |
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