PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra, Ivona VYCHODILOVÁ, Martina DVOŘÁČKOVÁ, Jana MAJERSKÁ, Ladislav DOKLÁDAL, Šárka SCHOŘOVÁ and Jiří FAJKUS. Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. The Plant Journal. Hoboken (USA): Blackwell Science, 2014, vol. 77, No 5, p. 770-781. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.12428. |
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@article{1199821, author = {Procházková Schrumpfová, Petra and Vychodilová, Ivona and Dvořáčková, Martina and Majerská, Jana and Dokládal, Ladislav and Schořová, Šárka and Fajkus, Jiří}, article_location = {Hoboken (USA)}, article_number = {5}, doi = {http://dx.doi.org/10.1111/tpj.12428}, keywords = {telomere protein interaction; telomere repeat binding (TRB); telomere; Arabidopsis thaliana; plant shelterin; telomerase}, language = {eng}, issn = {0960-7412}, journal = {The Plant Journal}, title = {Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase}, url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.12428/epdf}, volume = {77}, year = {2014} }
TY - JOUR ID - 1199821 AU - Procházková Schrumpfová, Petra - Vychodilová, Ivona - Dvořáčková, Martina - Majerská, Jana - Dokládal, Ladislav - Schořová, Šárka - Fajkus, Jiří PY - 2014 TI - Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase JF - The Plant Journal VL - 77 IS - 5 SP - 770-781 EP - 770-781 PB - Blackwell Science SN - 09607412 KW - telomere protein interaction KW - telomere repeat binding (TRB) KW - telomere KW - Arabidopsis thaliana KW - plant shelterin KW - telomerase UR - http://onlinelibrary.wiley.com/doi/10.1111/tpj.12428/epdf L2 - http://onlinelibrary.wiley.com/doi/10.1111/tpj.12428/epdf N2 - Although telomere-binding proteins constitute an essential part of telomeres, in vivo data indicating the existence of a structure similar to mammalian shelterin complex in plants are limited. Partial characterization of a number of candidate proteins has not identified true components of plant shelterin or elucidated their functional mechanisms. Telomere repeat binding (TRB) proteins from Arabidopsis thaliana bind plant telomeric repeats through a Myb domain of the telobox type in vitro, and have been shown to interact with POT1b (Protection of telomeres 1). Here we demonstrate co-localization of TRB1 protein with telomeres in situ using fluorescence microscopy, as well as in vivo interaction using chromatin immunoprecipitation. Classification of the TRB1 protein as a component of plant telomeres is further confirmed by the observation of shortening of telomeres in knockout mutants of the trb1 gene. Moreover, TRB proteins physically interact with plant telomerase catalytic subunits. These findings integrate TRB proteins into the telomeric interactome of A. thaliana. ER -
PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra, Ivona VYCHODILOVÁ, Martina DVOŘÁČKOVÁ, Jana MAJERSKÁ, Ladislav DOKLÁDAL, Šárka SCHOŘOVÁ and Jiří FAJKUS. Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. \textit{The Plant Journal}. Hoboken (USA): Blackwell Science, 2014, vol.~77, No~5, p.~770-781. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.12428.
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