Telomere repeat binding proteins are functional components of
Arabidopsis telomeres and interact with telomerase

J 2014

Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase

PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra, Ivona VYCHODILOVÁ, Martina DVOŘÁČKOVÁ, Jana MAJERSKÁ, Ladislav DOKLÁDAL et. al.

Basic information

Original name

Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase

Authors

PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra (203 Czech Republic, guarantor, belonging to the institution), Ivona VYCHODILOVÁ (203 Czech Republic, belonging to the institution), Martina DVOŘÁČKOVÁ (203 Czech Republic, belonging to the institution), Jana MAJERSKÁ (703 Slovakia, belonging to the institution), Ladislav DOKLÁDAL (203 Czech Republic, belonging to the institution), Šárka SCHOŘOVÁ (203 Czech Republic, belonging to the institution) and Jiří FAJKUS (203 Czech Republic, belonging to the institution)

Edition

The Plant Journal, Hoboken (USA), Blackwell Science, 2014, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.972

RIV identification code

RIV/00216224:14740/14:00073912

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/tpj.12428

UT WoS

000331848700010

Keywords in English

telomere protein interaction; telomere repeat binding (TRB); telomere; Arabidopsis thaliana; plant shelterin; telomerase

Abstract

V originále

Although telomere-binding proteins constitute an essential part of telomeres, in vivo data indicating the existence of a structure similar to mammalian shelterin complex in plants are limited. Partial characterization of a number of candidate proteins has not identified true components of plant shelterin or elucidated their functional mechanisms. Telomere repeat binding (TRB) proteins from Arabidopsis thaliana bind plant telomeric repeats through a Myb domain of the telobox type in vitro, and have been shown to interact with POT1b (Protection of telomeres 1). Here we demonstrate co-localization of TRB1 protein with telomeres in situ using fluorescence microscopy, as well as in vivo interaction using chromatin immunoprecipitation. Classification of the TRB1 protein as a component of plant telomeres is further confirmed by the observation of shortening of telomeres in knockout mutants of the trb1 gene. Moreover, TRB proteins physically interact with plant telomerase catalytic subunits. These findings integrate TRB proteins into the telomeric interactome of A. thaliana.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GA13-06943S, research and development project

Name: Strukturní a funkční komponenty rostlinných telomer

Investor: Czech Science Foundation

Citovat

PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra, Ivona VYCHODILOVÁ, Martina DVOŘÁČKOVÁ, Jana MAJERSKÁ, Ladislav DOKLÁDAL, Šárka SCHOŘOVÁ and Jiří FAJKUS. Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. The Plant Journal. Hoboken (USA): Blackwell Science, 2014, vol. 77, No 5, p. 770-781. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.12428.

@article{1199821,
   author = {Procházková Schrumpfová, Petra and Vychodilová, Ivona and Dvořáčková, Martina and Majerská, Jana and Dokládal, Ladislav and Schořová, Šárka and Fajkus, Jiří},
   article_location = {Hoboken (USA)},
   article_number = {5},
   doi = {http://dx.doi.org/10.1111/tpj.12428},
   keywords = {telomere protein interaction; telomere repeat binding (TRB); telomere; Arabidopsis thaliana; plant shelterin; telomerase},
   language = {eng},
   issn = {0960-7412},
   journal = {The Plant Journal},
   title = {Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.12428/epdf},
   volume = {77},
   year = {2014}
}

TY  - JOUR
ID  - 1199821
AU  - Procházková Schrumpfová, Petra - Vychodilová, Ivona - Dvořáčková, Martina - Majerská, Jana - Dokládal, Ladislav - Schořová, Šárka - Fajkus, Jiří
PY  - 2014
TI  - Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase
JF  - The Plant Journal
VL  - 77
IS  - 5
SP  - 770-781
EP  - 770-781
PB  - Blackwell Science
SN  - 09607412
KW  - telomere protein interaction
KW  - telomere repeat binding (TRB)
KW  - telomere
KW  - Arabidopsis thaliana
KW  - plant shelterin
KW  - telomerase
UR  - http://onlinelibrary.wiley.com/doi/10.1111/tpj.12428/epdf
L2  - http://onlinelibrary.wiley.com/doi/10.1111/tpj.12428/epdf
N2  - Although telomere-binding proteins constitute an essential part of telomeres, in vivo data indicating the existence of a structure similar to mammalian shelterin complex in plants are limited. Partial characterization of a number of candidate proteins has not identified true components of plant shelterin or elucidated their functional mechanisms. Telomere repeat binding (TRB) proteins from Arabidopsis thaliana bind plant telomeric repeats through a Myb domain of the telobox type in vitro, and have been shown to interact with POT1b (Protection of telomeres 1). Here we demonstrate co-localization of TRB1 protein with telomeres in situ using fluorescence microscopy, as well as in vivo interaction using chromatin immunoprecipitation. Classification of the TRB1 protein as a component of plant telomeres is further confirmed by the observation of shortening of telomeres in knockout mutants of the trb1 gene. Moreover, TRB proteins physically interact with plant telomerase catalytic subunits. These findings integrate TRB proteins into the telomeric interactome of A. thaliana.
ER  -

PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra, Ivona VYCHODILOVÁ, Martina DVOŘÁČKOVÁ, Jana MAJERSKÁ, Ladislav DOKLÁDAL, Šárka SCHOŘOVÁ and Jiří FAJKUS. Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. \textit{The Plant Journal}. Hoboken (USA): Blackwell Science, 2014, vol.~77, No~5, p.~770-781. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.12428.

Detailed Information on Publication Record