Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase

PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra, Ivona VYCHODILOVÁ, Martina DVOŘÁČKOVÁ, Jana MAJERSKÁ, Ladislav DOKLÁDAL, Šárka SCHOŘOVÁ and Jiří FAJKUS. Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. The Plant Journal. Hoboken (USA): Blackwell Science, 2014, vol. 77, No 5, p. 770-781. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.12428.

Basic information

• Original name: Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase
• Authors: PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra (203 Czech Republic, guarantor, belonging to the institution), Ivona VYCHODILOVÁ (203 Czech Republic, belonging to the institution), Martina DVOŘÁČKOVÁ (203 Czech Republic, belonging to the institution), Jana MAJERSKÁ (703 Slovakia, belonging to the institution), Ladislav DOKLÁDAL (203 Czech Republic, belonging to the institution), Šárka SCHOŘOVÁ (203 Czech Republic, belonging to the institution) and Jiří FAJKUS (203 Czech Republic, belonging to the institution).
• Edition: The Plant Journal, Hoboken (USA), Blackwell Science, 2014, 0960-7412.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 5.972
• RIV identification code: RIV/00216224:14740/14:00073912
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1111/tpj.12428
• UT WoS: 000331848700010
• Keywords in English: telomere protein interaction; telomere repeat binding (TRB); telomere; Arabidopsis thaliana; plant shelterin; telomerase
• Tags: kontrola MP, rivok
• Tags: International impact, Reviewed

Abstract

Although telomere-binding proteins constitute an essential part of telomeres, in vivo data indicating the existence of a structure similar to mammalian shelterin complex in plants are limited. Partial characterization of a number of candidate proteins has not identified true components of plant shelterin or elucidated their functional mechanisms. Telomere repeat binding (TRB) proteins from Arabidopsis thaliana bind plant telomeric repeats through a Myb domain of the telobox type in vitro, and have been shown to interact with POT1b (Protection of telomeres 1). Here we demonstrate co-localization of TRB1 protein with telomeres in situ using fluorescence microscopy, as well as in vivo interaction using chromatin immunoprecipitation. Classification of the TRB1 protein as a component of plant telomeres is further confirmed by the observation of shortening of telomeres in knockout mutants of the trb1 gene. Moreover, TRB proteins physically interact with plant telomerase catalytic subunits. These findings integrate TRB proteins into the telomeric interactome of A. thaliana.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• EE2.3.30.0009, research and development project: Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
• GA13-06943S, research and development project: Name: Strukturní a funkční komponenty rostlinných telomer

Investor: Czech Science Foundation