ALTERATIONS OF THIAMINE METABOLISM AND INCERASED
CABOXYMETHYLLYSINE LEVELS IN WOMEN WITH GESTATIONAL DIABETES
MELLITUS

k 2014

ALTERATIONS OF THIAMINE METABOLISM AND INCERASED CABOXYMETHYLLYSINE LEVELS IN WOMEN WITH GESTATIONAL DIABETES MELLITUS

BARTÁKOVÁ, Vendula, Anna PLESKAČOVÁ, Roman KUDLÁČ, Katarína KURICOVÁ, Veronika DVOŘÁKOVÁ et. al.

Basic information

Original name

ALTERATIONS OF THIAMINE METABOLISM AND INCERASED CABOXYMETHYLLYSINE LEVELS IN WOMEN WITH GESTATIONAL DIABETES MELLITUS

Name (in English)

ALTERATIONS OF THIAMINE METABOLISM AND INCERASED CABOXYMETHYLLYSINE LEVELS IN WOMEN WITH GESTATIONAL DIABETES MELLITUS

Authors

BARTÁKOVÁ, Vendula, Anna PLESKAČOVÁ, Roman KUDLÁČ, Katarína KURICOVÁ, Veronika DVOŘÁKOVÁ, Jana BĚLOBRÁDKOVÁ, Josef TOMANDL, Katarina ŠEBEKOVÁ, Lukáš PÁCAL and Kateřina KAŇKOVÁ

Edition

The 46th Annual Meeting of the Diabetes Pregnancy Study Group (DPSG), Budapest, Hungary, 2014

Other information

Type of outcome

Prezentace na konferencích

Confidentiality degree

není předmětem státního či obchodního tajemství

Keywords (in Czech)

gestační diabetes, thiamin, vitamín B1, AGEs, karboxy-metyl-lyzin

Keywords in English

gestational diabetes, thiamine, carboxymethyllyzine, pregnancy, vitamin B1

Změněno: 6/10/2014 15:19, MUDr. Vendula Bartáková, Ph.D.

Abstract

V originále

Thiamine (vitamin B1) - as a cofactor of several enzymes incl. transketolase (TKT) - participates in the regulation of glucose metabolism. Experimental evidence has shown thiamine disturbances in diabetes are potentially involved in the development and progression of diabetic microvascular complications since non-oxidative branch of pentose phosphate pathway (PPP) with TKT as a key enzyme supposedly plays a protective role. Data are lacking whether similar abnormalities may also play a role in the development of gestational diabetes mellitus (GDM). One of the consequences of thiamine deficiency might be accelerated formation of Advanced Glycation End products (AGEs). AGEs are traditionally considered as a result of long-term dysregulation of glucose homeostasis, however detailed kinetic studies indicate a prompt reflexion of glucose metabolism, therefore GDM should be an ideal phenotype to study acute changes. We aimed to determine plasma thiamine levels, erythrocyte thiamine diphosphate (TDP) levels (both by HPLC) transketolase activity (using kinetic method) and thiamine effect in pregnant women with and without GDM. Furthermore carboxymethyllysine (CML), the dominant form of AGEs in plasma, was determined by ELISA to find out a possible correlation with glucose metabolism and thiamine parameters. A total of 182 pregnant women (105 had GDM, 77 had physiologic pregnancy) were included in the study. Samples of peripheral blood were taken from each participant between 24th and 30th week of pregnancy and from 6 weeks to 12 months postpartum. Significant differences in plasma thiamine levels, erythrocyte TDP and TKT activity between GDM and physiologic pregnancy group were found in the 2nd trimester of pregnancy (P=0.01, P<0.001 and P=0.02, respectively, Mann-Whitney test) while no significant differences were found postpartum (all three P0.05, Mann-Whitney test). Plasma CML levels were significantly higher in GDM compared to normal pregnancy in 2nd trimester (P=0.02 resp., Mann-Whitney test). Plasma thiamine as well as CML levels in 2nd trimester significantly inversely correlated with pre-gestational BMI (P<0.05, Spearman) but not with age or glycaemias during oGTT test. In conclusion, we found statistically significant increase of plasma CML levels in pregnant women with GDM in the 2nd trimester of gravidity without concomitant alteration of parameters of thiamine metabolism. Therefore activity of PPP does not seem to play a protective role against the formation of AGEs. The relevance of their increased levels for both mother and child warrants further investigation. Supported by IGA MZ ČR grant, no. NT11405

Links

NT11405, research and development project

Name: Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu

Investor: Ministry of Health of the CR

Citovat

BARTÁKOVÁ, Vendula, Anna PLESKAČOVÁ, Roman KUDLÁČ, Katarína KURICOVÁ, Veronika DVOŘÁKOVÁ, Jana BĚLOBRÁDKOVÁ, Josef TOMANDL, Katarina ŠEBEKOVÁ, Lukáš PÁCAL and Kateřina KAŇKOVÁ. ALTERATIONS OF THIAMINE METABOLISM AND INCERASED CABOXYMETHYLLYSINE LEVELS IN WOMEN WITH GESTATIONAL DIABETES MELLITUS. In The 46th Annual Meeting of the Diabetes Pregnancy Study Group (DPSG), Budapest, Hungary. 2014.

@proceedings{1201861,
   author = {Bartáková, Vendula and Pleskačová, Anna and Kudláč, Roman and Kuricová, Katarína and Dvořáková, Veronika and Bělobrádková, Jana and Tomandl, Josef and Šebeková, Katarina and Pácal, Lukáš and Kaňková, Kateřina},
   booktitle = {The 46th Annual Meeting of the Diabetes Pregnancy Study Group (DPSG), Budapest, Hungary},
   keywords = {gestational diabetes, thiamine, carboxymethyllyzine, pregnancy, vitamin B1},
   title = {ALTERATIONS OF THIAMINE METABOLISM AND INCERASED CABOXYMETHYLLYSINE LEVELS IN WOMEN WITH GESTATIONAL DIABETES MELLITUS},
   year = {2014}
}

TY  - CONF
ID  - 1201861
AU  - Bartáková, Vendula - Pleskačová, Anna - Kudláč, Roman - Kuricová, Katarína - Dvořáková, Veronika - Bělobrádková, Jana - Tomandl, Josef - Šebeková, Katarina - Pácal, Lukáš - Kaňková, Kateřina
PY  - 2014
TI  - ALTERATIONS OF THIAMINE METABOLISM AND INCERASED CABOXYMETHYLLYSINE LEVELS IN WOMEN WITH GESTATIONAL DIABETES MELLITUS
KW  - gestational diabetes, thiamine, carboxymethyllyzine, pregnancy, vitamin B1
N2  - Thiamine (vitamin B1) - as a cofactor of several enzymes incl. transketolase (TKT) - participates in the regulation of glucose metabolism. Experimental evidence has shown thiamine disturbances in diabetes are potentially involved in the development and progression of diabetic microvascular complications since non-oxidative branch of pentose phosphate pathway (PPP) with TKT as a key enzyme supposedly plays a protective role. Data are lacking whether similar abnormalities may also play a role in the development of gestational diabetes mellitus (GDM). One of the consequences of thiamine deficiency might be accelerated formation of Advanced Glycation End products (AGEs). AGEs are traditionally considered as a result of long-term dysregulation of glucose homeostasis, however detailed kinetic studies indicate a prompt reflexion of glucose metabolism, therefore GDM should be an ideal phenotype to study acute changes. We aimed to determine plasma thiamine levels, erythrocyte thiamine diphosphate (TDP) levels (both by HPLC) transketolase activity (using kinetic method) and thiamine effect in pregnant women with and without GDM. Furthermore carboxymethyllysine (CML), the dominant form of AGEs in plasma, was determined by ELISA to find out a possible correlation with glucose metabolism and thiamine parameters. A total of 182 pregnant women (105 had GDM, 77 had physiologic pregnancy) were included in the study. Samples of peripheral blood were taken from each participant between 24th and 30th week of pregnancy and from 6 weeks to 12 months postpartum. Significant differences in plasma thiamine levels, erythrocyte TDP and TKT activity between GDM and physiologic pregnancy group were found in the 2nd trimester of pregnancy (P=0.01, P<0.001 and P=0.02, respectively, Mann-Whitney test) while no significant differences were found postpartum (all three P0.05, Mann-Whitney test). Plasma CML levels were significantly higher in GDM compared to normal pregnancy in 2nd trimester (P=0.02 resp., Mann-Whitney test). Plasma thiamine as well as CML levels in 2nd trimester significantly inversely correlated with pre-gestational BMI (P<0.05, Spearman) but not with age or glycaemias during oGTT test. In conclusion, we found statistically significant increase of plasma CML levels in pregnant women with GDM in the 2nd trimester of gravidity without concomitant alteration of parameters of thiamine metabolism. Therefore activity of PPP does not seem to play a protective role against the formation of AGEs. The relevance of their increased levels for both mother and child warrants further investigation. Supported by IGA MZ ČR grant, no. NT11405
ER  -

BARTÁKOVÁ, Vendula, Anna PLESKAČOVÁ, Roman KUDLÁČ, Katarína KURICOVÁ, Veronika DVOŘÁKOVÁ, Jana BĚLOBRÁDKOVÁ, Josef TOMANDL, Katarina ŠEBEKOVÁ, Lukáš PÁCAL and Kateřina KAŇKOVÁ. ALTERATIONS OF THIAMINE METABOLISM AND INCERASED CABOXYMETHYLLYSINE LEVELS IN WOMEN WITH GESTATIONAL DIABETES MELLITUS. In \textit{The 46th Annual Meeting of the Diabetes Pregnancy Study Group (DPSG), Budapest, Hungary}. 2014.

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