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@proceedings{1201893,
author = {Vaškovicová, Naděžda and Valigurová, Andrea and Hodová, Iveta and Melicherová, Janka and Krzyžánek, Vladislav},
booktitle = {The 18th International Microscopy Congress},
keywords = {freeze-fracture technique; artefacts; cryoprotectant; fragmentation; morphological structures},
language = {eng},
isbn = {978-80-260-6720-7},
title = {Freezer-fracture technique and artefacts caused by processing conditions.},
year = {2014}
}
TY - CONF
ID - 1201893
AU - Vaškovicová, Naděžda - Valigurová, Andrea - Hodová, Iveta - Melicherová, Janka - Krzyžánek, Vladislav
PY - 2014
TI - Freezer-fracture technique and artefacts caused by processing conditions.
SN - 9788026067207
KW - freeze-fracture technique
KW - artefacts
KW - cryoprotectant
KW - fragmentation
KW - morphological structures
N2 - Freeze-fracture technique is method for visualisation membrane surfaces of cell organelles. This method used cryo-fixation for stabilization a sample. Sample is frozen in nitrogen and cut in the chamber under a vacuum and low temperature. Glycerol is used as a cryoprotectant for observation fine and smooth cellular structures. Although, cryoprotectants serve for the substitution water and elimination ice crystals production, they could affect a form of fracture through biological membranes and could affect interpretation of morphological structures.
ER -
VAŠKOVICOVÁ, Naděžda, Andrea VALIGUROVÁ, Iveta HODOVÁ, Janka MELICHEROVÁ and Vladislav KRZYŽÁNEK. Freezer-fracture technique and artefacts caused by processing conditions. In \textit{The 18th International Microscopy Congress}. 2014. ISBN~978-80-260-6720-7.
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