| DUBSKÁ, Lenka, Eva MATALOVÁ a Ivan MÍŠEK. Detection of apoptosis in paraffin embedded tissues: the influence of tissue type and fixation. Online. Acta veterinaria (Brno). Brno: VFU Brno, ČR, 2002, roč. 71, č. 4, s. 529-533. ISSN 0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb200271040529. [citováno 2024-04-23] |
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@article{1202695,
author = {Dubská, Lenka and Matalová, Eva and Míšek, Ivan},
article_location = {Brno},
article_number = {4},
doi = {http://dx.doi.org/10.2754/avb200271040529},
issn = {0001-7213},
journal = {Acta veterinaria (Brno)},
title = {Detection of apoptosis in paraffin embedded tissues: the influence of tissue type and fixation},
volume = {71},
year = {2002}
}
TY - JOUR
ID - 1202695
AU - Dubská, Lenka - Matalová, Eva - Míšek, Ivan
PY - 2002
TI - Detection of apoptosis in paraffin embedded tissues: the influence of tissue type and fixation
JF - Acta veterinaria (Brno)
VL - 71
IS - 4
SP - 529-533
EP - 529-533
PB - VFU Brno, ČR
SN - 00017213
N2 - With increasing importance of apoptosis, many assays have been developed to detect apoptosis. in cell populations and individual cells. Apoptotic cells are often proved using TUNEL assay based on detection of DNA strand breaks that occur during apoptosis. The method enables quantification of apoptotic cells by flow cytometry, fluorescence as well as by light microscopy when modifications using marker enzymes are applied. Disadvantages of this method are false positive and negative findings. In this study, the influence of different methods of fixation on TUNEL POD results was investigated, Three commonly employed fixative procedures using formaldehyde, methacarn and Bouin's fluid were applied. Two bovine tissue types of different cell density (thymus and ovary) were used to test this effect. Different pretreatments were tested for an influence upon false negative findings. Results were evaluated under light microscopy and photographed. We observed that, in the absence of a universal molecular marker of programmed cell death, it is essential for reliable apoptosis detection to optimize the chosen method for a given tissue and fixation. It is also strongly recommended to employ at least two methods, measuring different features of the apoptotic process to obtain reproducible results in detection of apoptosis.
ER -
DUBSKÁ, Lenka, Eva MATALOVÁ a Ivan MÍŠEK. Detection of apoptosis in paraffin embedded tissues: the influence of tissue type and fixation. Online. \textit{Acta veterinaria (Brno)}. Brno: VFU Brno, ČR, 2002, roč.~71, č.~4, s.~529-533. ISSN~0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb200271040529. [citováno 2024-04-23]
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