Haematopoietic developmental potential of human pluripotent
stem cell lines

J 2014

Haematopoietic developmental potential of human pluripotent stem cell lines

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ

Basic information

Original name

Haematopoietic developmental potential of human pluripotent stem cell lines

Authors

TESAŘOVÁ, Lenka (203 Czech Republic, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Stanislav STEJSKAL (203 Czech Republic, belonging to the institution) and Irena KRONTORÁD KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Folia biologica, Praha, Univerzita Karlova, 2014, 0015-5500

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.000

RIV identification code

RIV/00216224:14330/14:00073970

Organization unit

Faculty of Informatics

UT WoS

000343275800015

Keywords (in Czech)

lidské pluripotentní kmenové buňky; hematopoetický vývoj; embryoidní tělíska; CD34+ buňky

Keywords in English

human pluripotent stem cells; hematopoietic development; embryoid bodies; CD34+ cells

Abstract

V originále

The generation of hematopoietic progenitors from human pluripotent stem cells (hPSCs) presents great promise for cell-replacement therapies. However, current protocols for hematopoietic differentiation of hPSCs suffer from low efficiency and functional defects in the derived cells. The technology is also limited by variable ability of hPSC lines to generate blood cells in vitro. To address this issue, methodologies for hematopoietic differentiation in feeder-free conditions were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines in this study. It was found that these cell lines did not generate hematopoietic progenitors in such an extent as H1 and H9 hESC lines used for this purpose in the vast majority of relevant studies. These results suggest that for clinical application of blood cells derived from hPSCs, possibly from autologous hiPSCs, it is necessary to overcome the variability in the hematopoietic developmental potential of individual hPSC lines.

Links

CZ.1.07/2.3.00/30.0030, interní kód MU

Name: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ. Haematopoietic developmental potential of human pluripotent stem cell lines. Folia biologica. Praha: Univerzita Karlova, 2014, vol. 60, No 1, p. 90-94. ISSN 0015-5500.

@article{1202839,
   author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena},
   article_location = {Praha},
   article_number = {1},
   keywords = {human pluripotent stem cells; hematopoietic development; embryoid bodies; CD34+ cells},
   language = {eng},
   issn = {0015-5500},
   journal = {Folia biologica},
   title = {Haematopoietic developmental potential of human pluripotent stem cell lines},
   volume = {60},
   year = {2014}
}

TY  - JOUR
ID  - 1202839
AU  - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena
PY  - 2014
TI  - Haematopoietic developmental potential of human pluripotent stem cell lines
JF  - Folia biologica
VL  - 60
IS  - 1
SP  - 90-94
EP  - 90-94
PB  - Univerzita Karlova
SN  - 00155500
KW  - human pluripotent stem cells
KW  - hematopoietic development
KW  - embryoid bodies
KW  - CD34+ cells
N2  - The generation of hematopoietic progenitors from human pluripotent stem cells (hPSCs) presents great promise for cell-replacement therapies. However, current protocols for hematopoietic differentiation of hPSCs suffer from low efficiency and functional defects in the derived cells. The technology is also limited by variable ability of hPSC lines to generate blood cells in vitro. To address this issue, methodologies for hematopoietic differentiation in feeder-free conditions were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines in this study. It was found that these cell lines did not generate hematopoietic progenitors in such an extent as H1 and H9 hESC lines used for this purpose in the vast majority of relevant studies. These results suggest that for clinical application of blood cells derived from hPSCs, possibly from autologous hiPSCs, it is necessary to overcome the variability in the hematopoietic developmental potential of individual hPSC lines.
ER  -

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ. Haematopoietic developmental potential of human pluripotent stem cell lines. \textit{Folia biologica}. Praha: Univerzita Karlova, 2014, vol.~60, No~1, p.~90-94. ISSN~0015-5500.

Detailed Information on Publication Record