TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ. Haematopoietic developmental potential of human pluripotent stem cell lines. Folia biologica. Praha: Univerzita Karlova, 2014, vol. 60, No 1, p. 90-94. ISSN 0015-5500. |
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@article{1202839, author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena}, article_location = {Praha}, article_number = {1}, keywords = {human pluripotent stem cells; hematopoietic development; embryoid bodies; CD34+ cells}, language = {eng}, issn = {0015-5500}, journal = {Folia biologica}, title = {Haematopoietic developmental potential of human pluripotent stem cell lines}, volume = {60}, year = {2014} }
TY - JOUR ID - 1202839 AU - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena PY - 2014 TI - Haematopoietic developmental potential of human pluripotent stem cell lines JF - Folia biologica VL - 60 IS - 1 SP - 90-94 EP - 90-94 PB - Univerzita Karlova SN - 00155500 KW - human pluripotent stem cells KW - hematopoietic development KW - embryoid bodies KW - CD34+ cells N2 - The generation of hematopoietic progenitors from human pluripotent stem cells (hPSCs) presents great promise for cell-replacement therapies. However, current protocols for hematopoietic differentiation of hPSCs suffer from low efficiency and functional defects in the derived cells. The technology is also limited by variable ability of hPSC lines to generate blood cells in vitro. To address this issue, methodologies for hematopoietic differentiation in feeder-free conditions were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines in this study. It was found that these cell lines did not generate hematopoietic progenitors in such an extent as H1 and H9 hESC lines used for this purpose in the vast majority of relevant studies. These results suggest that for clinical application of blood cells derived from hPSCs, possibly from autologous hiPSCs, it is necessary to overcome the variability in the hematopoietic developmental potential of individual hPSC lines. ER -
TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ. Haematopoietic developmental potential of human pluripotent stem cell lines. \textit{Folia biologica}. Praha: Univerzita Karlova, 2014, vol.~60, No~1, p.~90-94. ISSN~0015-5500.
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