LITERÁK, Ivan, B SMID, Lenka ZDRAŽILOVÁ DUBSKÁ, L BRYNDZA a Lubomír VALÍČEK. An outbreak of the polyomavirus infection in budgerigars and cockatiels in Slovakia, including a genome analysis of an avian polyomavirus isolate. AVIAN DISEASES. ATHENS: AMER ASSOC AVIAN PATHOLOGISTS, roč. 50, č. 1, s. 120-123. ISSN 0005-2086. doi:10.1637/7395-061605R.1. 2006.
Další formáty:   BibTeX LaTeX RIS
Základní údaje
Originální název An outbreak of the polyomavirus infection in budgerigars and cockatiels in Slovakia, including a genome analysis of an avian polyomavirus isolate
Autoři LITERÁK, Ivan, B SMID, Lenka ZDRAŽILOVÁ DUBSKÁ, L BRYNDZA a Lubomír VALÍČEK.
Vydání AVIAN DISEASES, ATHENS, AMER ASSOC AVIAN PATHOLOGISTS, 2006, 0005-2086.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 1.369
Doi http://dx.doi.org/10.1637/7395-061605R.1
UT WoS 000236054400022
Klíčová slova anglicky bird; APV; Mellopsitacus; Nymphicus; electron microscopy; DNA analysis
Změnil Změnil: MUDr. RNDr. Michal Řiháček, Ph.D., EuSpLM, učo 357305. Změněno: 21. 10. 2014 11:51.
Anotace
In winter 2003-04, large numbers of budgerigars (Mellopsitacus undulatus) and cockatiels (Nymphicus hollandicus) fell ill and died in a large parrot-breeding aviary in Slovakia. In budgerigars, the disease outbreak occurred at the age of 2-3 weeks; cockatiels died within their first 7 days of life. In budgerigars, symptoms of the disease included delayed growth, tremor, darkish discoloration of skin, quill bleeding, and feathering defects. cockatiels often died without any symptoms and with a full crop; feathering defects occurred sporadically. Electron microscopy with negative staining of aqueous lysates of the affected skin and of bleeding quills showed isolated or Clustered polyomavirus particles 45-50 nm in size. Long filamentous forms of the virus were also found in virion Clusters of skin lysates from the budgerigars. In ultrathin sections through the pathologically altered skin tissue of budgerigars, virus particles were present in both nuclei and cytoplasm of epidermal cells, often in crystalline form. In infected cells, enlarged nuclei showed an extensive chromatin margination. On the DNA level, presence of a polyomavirus infection was conclusively proved by the polymerase chain reaction using avian polyomavirus (APV)-specific primers. A sequence analysis of the gene encoding viral protein (VP)1 and of the combined region for VP2 and VP3 proteins revealed a previously undescribed synonymous mutation in this isolate. This report extended the knowledge of the area of APV Occurrence and of the spectrum of hosts in the context of genomic and morphologic variability of APV isolates.
VytisknoutZobrazeno: 28. 3. 2024 14:06