HPLC-MS Identification of Metabolites of Selected
Benzophenanthridine Alkaloids by Rat Liver Microsomes

D 2014

HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes

MIDLIK, Adam, Kristýna ŠEBRLOVÁ, Ivana KUŠNYEROVÁ, Gabriela DOVRTĚLOVÁ, Kristýna NOSKOVÁ et. al.

Basic information

Original name

HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes

Authors

MIDLIK, Adam, Kristýna ŠEBRLOVÁ, Ivana KUŠNYEROVÁ, Gabriela DOVRTĚLOVÁ, Kristýna NOSKOVÁ, Jan JUŘICA, Eva TÁBORSKÁ and Ondřej PEŠ

Edition

Praha, Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech - Slovak Spectroscopic Conference, p. 205-474, 2014

Publisher

Ioannes Marcus Marci Spectroscopic Society

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

storage medium (CD, DVD, flash disk)

Organization unit

Faculty of Medicine

ISBN

978-80-905704-1-2

Keywords in English

LC MS; benzophenanthridine alkaloids; metabolites; microsomes

Změněno: 7/11/2014 08:22, doc. Mgr. Ondřej Peš, Ph.D.

Abstract

V originále

Quaternary benzophenanthridine alkaloids (QBAs) are a group of isoquinoline derivatives produced by plants of the Papaveraceae, Fumariaceae and Rutaceae family. Presently they are studied intensively because of their biological activity. Anti-inflammatory, anti-microbial, anti-fungal, antiparasitic, as well as pro-apoptotic and anti-tumor effects of QBAs have been reported. Determination of toxicity of these potential drugs is based on metabolic studies of major, commercially available sanguinarine (SA) and chelerythrine (CHE). However, metabolic fate and pharmacological activity of minor QBAs, such as chelilutine (CL), chelirubine (CR), sanguilutine (SL), sanguirubine (SR) and macarpine (MA), might differ considerably, despite their structural similarity. Rat liver microsomes (RLMs), subcellular particles containing metabolizing enzymes, such as a cytochrome P450 family, provide a simple metabolic model for in vitro investigation before carrying out in vivo tests.

Links

LH12176, research and development project

Name: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/0818/2012, interní kód MU

Name: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: BIOMET 2)

Investor: Masaryk University, Category A

Citovat

MIDLIK, Adam, Kristýna ŠEBRLOVÁ, Ivana KUŠNYEROVÁ, Gabriela DOVRTĚLOVÁ, Kristýna NOSKOVÁ, Jan JUŘICA, Eva TÁBORSKÁ and Ondřej PEŠ. HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes. In Vítězslav Otruba, Michaela Vašinová Galiová. Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech - Slovak Spectroscopic Conference. Praha: Ioannes Marcus Marci Spectroscopic Society, 2014, p. 205-474. ISBN 978-80-905704-1-2.

@inproceedings{1204445,
   author = {Midlik, Adam and Šebrlová, Kristýna and Kušnyerová, Ivana and Dovrtělová, Gabriela and Nosková, Kristýna and Juřica, Jan and Táborská, Eva and Peš, Ondřej},
   address = {Praha},
   booktitle = {Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 & 15th Czech - Slovak Spectroscopic Conference},
   editor = {Vítězslav Otruba, Michaela Vašinová Galiová},
   keywords = {LC MS; benzophenanthridine alkaloids; metabolites; microsomes},
   howpublished = {paměťový nosič},
   language = {eng},
   location = {Praha},
   isbn = {978-80-905704-1-2},
   pages = {205-474},
   publisher = {Ioannes Marcus Marci Spectroscopic Society},
   title = {HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes},
   year = {2014}
}

TY  - JOUR
ID  - 1204445
AU  - Midlik, Adam - Šebrlová, Kristýna - Kušnyerová, Ivana - Dovrtělová, Gabriela - Nosková, Kristýna - Juřica, Jan - Táborská, Eva - Peš, Ondřej
PY  - 2014
TI  - HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes
PB  - Ioannes Marcus Marci Spectroscopic Society
CY  - Praha
SN  - 9788090570412
KW  - LC MS
KW  - benzophenanthridine alkaloids
KW  - metabolites
KW  - microsomes
N2  - Quaternary benzophenanthridine alkaloids (QBAs) are a group of isoquinoline derivatives produced by plants of the Papaveraceae, Fumariaceae and Rutaceae family. Presently they are studied intensively because of their biological activity. Anti-inflammatory, anti-microbial, anti-fungal, antiparasitic, as well as pro-apoptotic and anti-tumor effects of QBAs have been reported. Determination of toxicity of these potential drugs is based on metabolic studies of major, commercially available sanguinarine (SA) and chelerythrine (CHE). However, metabolic fate and pharmacological activity of minor QBAs, such as chelilutine (CL), chelirubine (CR), sanguilutine (SL), sanguirubine (SR) and macarpine (MA), might differ considerably, despite their structural similarity. Rat liver microsomes (RLMs), subcellular particles containing metabolizing enzymes, such as a cytochrome P450 family, provide a simple metabolic model for in vitro investigation before carrying out in vivo tests.
ER  -

MIDLIK, Adam, Kristýna ŠEBRLOVÁ, Ivana KUŠNYEROVÁ, Gabriela DOVRTĚLOVÁ, Kristýna NOSKOVÁ, Jan JUŘICA, Eva TÁBORSKÁ and Ondřej PEŠ. HPLC-MS Identification of Metabolites of Selected Benzophenanthridine Alkaloids by Rat Liver Microsomes. In Vítězslav Otruba, Michaela Vašinová Galiová. \textit{Book of Abstracts European Symposium on Atomic Spectrometry ESAS 2014 \&{} 15th Czech - Slovak Spectroscopic Conference}. Praha: Ioannes Marcus Marci Spectroscopic Society, 2014, p.~205-474. ISBN~978-80-905704-1-2.

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