A Versatile Scaffold Contributes to Damage Survival via
Sumoylation and Nuclease Interactions

J 2014

A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

SARANGI, Prabha; Veronika ALTMANNOVÁ; Cory HOLLAND; Zdenka BARTOŠOVÁ; Fanfan HAO et. al.

Základní údaje

Originální název

A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

Autoři

SARANGI, Prabha (840 Spojené státy); Veronika ALTMANNOVÁ (203 Česká republika, domácí); Cory HOLLAND (840 Spojené státy); Zdenka BARTOŠOVÁ (703 Slovensko, domácí); Fanfan HAO (840 Spojené státy); Dorothea ANRATHER (40 Rakousko); Gustav AMMERER (40 Rakousko); Sang Eun LEE (840 Spojené státy); Lumír KREJČÍ (203 Česká republika, garant, domácí) a Xiaolan ZHAO (840 Spojené státy)

Vydání

Cell Reports, CAMBRIDGE, Spojené státy americké, Cell Press, 2014, 2211-1247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 8.358

Kód RIV

RIV/00216224:14110/14:00074018

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.celrep.2014.08.054

UT WoS

000344468100014

EID Scopus

2-s2.0-84907965952

Klíčová slova anglicky

STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 1. 2015 00:06, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.

Návaznosti

EE2.3.20.0011, projekt VaV

Název: Centrum výzkumu pluripotentních buněk a nestability genomu

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

Citovat

SARANGI, Prabha; Veronika ALTMANNOVÁ; Cory HOLLAND; Zdenka BARTOŠOVÁ; Fanfan HAO; Dorothea ANRATHER; Gustav AMMERER; Sang Eun LEE; Lumír KREJČÍ a Xiaolan ZHAO. A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions. Cell Reports. CAMBRIDGE, Spojené státy americké: Cell Press, 2014, roč. 9, č. 1, s. 143-152. ISSN 2211-1247. Dostupné z: https://doi.org/10.1016/j.celrep.2014.08.054.

@article{1204451,
   author = {Sarangi, Prabha and Altmannová, Veronika and Holland, Cory and Bartošová, Zdenka and Hao, Fanfan and Anrather, Dorothea and Ammerer, Gustav and Lee, Sang Eun and Krejčí, Lumír and Zhao, Xiaolan},
   article_location = {CAMBRIDGE, Spojené státy americké},
   article_number = {1},
   doi = {https://doi.org/10.1016/j.celrep.2014.08.054},
   keywords = {STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE},
   language = {eng},
   issn = {2211-1247},
   journal = {Cell Reports},
   title = {A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1204451
AU  - Sarangi, Prabha - Altmannová, Veronika - Holland, Cory - Bartošová, Zdenka - Hao, Fanfan - Anrather, Dorothea - Ammerer, Gustav - Lee, Sang Eun - Krejčí, Lumír - Zhao, Xiaolan
PY  - 2014
TI  - A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions
JF  - Cell Reports
VL  - 9
IS  - 1
SP  - 143-152
EP  - 143-152
PB  - Cell Press
SN  - 22111247
KW  - STRAND BREAK REPAIR
KW  - YEAST SCHIZOSACCHAROMYCES-POMBE
KW  - HOLLIDAY JUNCTION RESOLVASE
KW  - DNA-REPAIR
KW  - SACCHAROMYCES-CEREVISIAE
KW  - PROTEIN INTERACTIONS
KW  - RAD1-RAD10 NUCLEASE
KW  - ABASIC SITES
KW  - RECOMBINATION
KW  - ENDONUCLEASE
N2  - DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
ER  -

SARANGI, Prabha; Veronika ALTMANNOVÁ; Cory HOLLAND; Zdenka BARTOŠOVÁ; Fanfan HAO; Dorothea ANRATHER; Gustav AMMERER; Sang Eun LEE; Lumír KREJČÍ a Xiaolan ZHAO. A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions. \textit{Cell Reports}. CAMBRIDGE, Spojené státy americké: Cell Press, 2014, roč.~9, č.~1, s.~143-152. ISSN~2211-1247. Dostupné z: https://doi.org/10.1016/j.celrep.2014.08.054.

Přehled o publikaci