A Versatile Scaffold Contributes to Damage Survival via
Sumoylation and Nuclease Interactions

J 2014

A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

SARANGI, Prabha, Veronika ALTMANNOVÁ, Cory HOLLAND, Zdenka BARTOŠOVÁ, Fanfan HAO et. al.

Basic information

Original name

A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

Authors

SARANGI, Prabha (840 United States of America), Veronika ALTMANNOVÁ (203 Czech Republic, belonging to the institution), Cory HOLLAND (840 United States of America), Zdenka BARTOŠOVÁ (703 Slovakia, belonging to the institution), Fanfan HAO (840 United States of America), Dorothea ANRATHER (40 Austria), Gustav AMMERER (40 Austria), Sang Eun LEE (840 United States of America), Lumír KREJČÍ (203 Czech Republic, guarantor, belonging to the institution) and Xiaolan ZHAO (840 United States of America)

Edition

Cell Reports, CAMBRIDGE, Spojené státy americké, Cell Press, 2014, 2211-1247

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 8.358

RIV identification code

RIV/00216224:14110/14:00074018

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.celrep.2014.08.054

UT WoS

000344468100014

Keywords in English

STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE

Abstract

V originále

DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.

Links

EE2.3.20.0011, research and development project

Name: Centrum výzkumu pluripotentních buněk a nestability genomu

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP207/12/2323, research and development project

Name: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Czech Science Foundation

GA13-26629S, research and development project

Name: SUMO a stability genomu

Investor: Czech Science Foundation

Citovat

SARANGI, Prabha, Veronika ALTMANNOVÁ, Cory HOLLAND, Zdenka BARTOŠOVÁ, Fanfan HAO, Dorothea ANRATHER, Gustav AMMERER, Sang Eun LEE, Lumír KREJČÍ and Xiaolan ZHAO. A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions. Cell Reports. CAMBRIDGE, Spojené státy americké: Cell Press, 2014, vol. 9, No 1, p. 143-152. ISSN 2211-1247. Available from: https://dx.doi.org/10.1016/j.celrep.2014.08.054.

@article{1204451,
   author = {Sarangi, Prabha and Altmannová, Veronika and Holland, Cory and Bartošová, Zdenka and Hao, Fanfan and Anrather, Dorothea and Ammerer, Gustav and Lee, Sang Eun and Krejčí, Lumír and Zhao, Xiaolan},
   article_location = {CAMBRIDGE, Spojené státy americké},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.celrep.2014.08.054},
   keywords = {STRAND BREAK REPAIR; YEAST SCHIZOSACCHAROMYCES-POMBE; HOLLIDAY JUNCTION RESOLVASE; DNA-REPAIR; SACCHAROMYCES-CEREVISIAE; PROTEIN INTERACTIONS; RAD1-RAD10 NUCLEASE; ABASIC SITES; RECOMBINATION; ENDONUCLEASE},
   language = {eng},
   issn = {2211-1247},
   journal = {Cell Reports},
   title = {A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1204451
AU  - Sarangi, Prabha - Altmannová, Veronika - Holland, Cory - Bartošová, Zdenka - Hao, Fanfan - Anrather, Dorothea - Ammerer, Gustav - Lee, Sang Eun - Krejčí, Lumír - Zhao, Xiaolan
PY  - 2014
TI  - A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions
JF  - Cell Reports
VL  - 9
IS  - 1
SP  - 143-152
EP  - 143-152
PB  - Cell Press
SN  - 22111247
KW  - STRAND BREAK REPAIR
KW  - YEAST SCHIZOSACCHAROMYCES-POMBE
KW  - HOLLIDAY JUNCTION RESOLVASE
KW  - DNA-REPAIR
KW  - SACCHAROMYCES-CEREVISIAE
KW  - PROTEIN INTERACTIONS
KW  - RAD1-RAD10 NUCLEASE
KW  - ABASIC SITES
KW  - RECOMBINATION
KW  - ENDONUCLEASE
N2  - DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
ER  -

SARANGI, Prabha, Veronika ALTMANNOVÁ, Cory HOLLAND, Zdenka BARTOŠOVÁ, Fanfan HAO, Dorothea ANRATHER, Gustav AMMERER, Sang Eun LEE, Lumír KREJČÍ and Xiaolan ZHAO. A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions. \textit{Cell Reports}. CAMBRIDGE, Spojené státy americké: Cell Press, 2014, vol.~9, No~1, p.~143-152. ISSN~2211-1247. Available from: https://dx.doi.org/10.1016/j.celrep.2014.08.054.

Detailed Information on Publication Record