ŠIMARA, Pavel, Lenka TESAŘOVÁ, Stanislava PAĎOUROVÁ and Irena KRONTORÁD KOUTNÁ. Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods. Folia Biologica. Praha: Univerzita Karlova, 2014, vol. 60, S1, p. 85-89. ISSN 0015-5500. |
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@article{1204941, author = {Šimara, Pavel and Tesařová, Lenka and Paďourová, Stanislava and Krontorád Koutná, Irena}, article_location = {Praha}, article_number = {S1}, keywords = {hiPSCs; lentivirus; Sendai virus; episomal reprogramming}, language = {eng}, issn = {0015-5500}, journal = {Folia Biologica}, title = {Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods}, volume = {60}, year = {2014} }
TY - JOUR ID - 1204941 AU - Šimara, Pavel - Tesařová, Lenka - Paďourová, Stanislava - Krontorád Koutná, Irena PY - 2014 TI - Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods JF - Folia Biologica VL - 60 IS - S1 SP - 85-89 EP - 85-89 PB - Univerzita Karlova SN - 00155500 KW - hiPSCs KW - lentivirus KW - Sendai virus KW - episomal reprogramming N2 - Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfil this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during seven years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSC clones are highly preferable in terms of future clinical application. In this commentary article, we summarize the reprogramming experiments performed in our laboratories. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human neonatal fibroblasts and CD34+ blood progenitors were used as cell sources and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions. ER -
ŠIMARA, Pavel, Lenka TESAŘOVÁ, Stanislava PAĎOUROVÁ and Irena KRONTORÁD KOUTNÁ. Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods. \textit{Folia Biologica}. Praha: Univerzita Karlova, 2014, vol.~60, S1, p.~85-89. ISSN~0015-5500.
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