H3K56ac distribution in cancer and embryonic cell lines during cell cycle

STEJSKAL, Stanislav, Karel STEJSKAL, Karel ŠTĚPKA, Martina MATĚJKOVÁ, Zbyněk ZDRÁHAL and Irena KRONTORÁD KOUTNÁ. H3K56ac distribution in cancer and embryonic cell lines during cell cycle. In EMBO Stem Cells in Cancer and Regenerative Medicine. 2014.

Basic information

• Original name: H3K56ac distribution in cancer and embryonic cell lines during cell cycle
• Authors: STEJSKAL, Stanislav, Karel STEJSKAL, Karel ŠTĚPKA, Martina MATĚJKOVÁ, Zbyněk ZDRÁHAL and Irena KRONTORÁD KOUTNÁ.
• Edition: EMBO Stem Cells in Cancer and Regenerative Medicine, 2014.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Informatics
• Keywords in English: H3K56ac; cancer cells; cell cycle

Abstract

Acetylation of lysine 56 on histone H3 (H3K56ac) is very rare posttranslational modification in mammalian cells. This H3 core modification increases nucleosome breathing and promotes recruitment of chromatin remodeling complexes and consequently activates gene expression. Elevated level of H3K56ac was observed in cancer and stem cells especially during S and G2 phase of cell cycle. In this study we evaluated if this H3K56ac enhancement is connected with dysregulation of acetylation pathway or simply with increased level of cells in S and G2 phase within the cell population. To determine the role of H3K56ac level during cell cycle in cancer and stem cells we used different approaches (immunohistochemistry, western blot or mass spectrometry).

Links

• CZ.1.05/1.1.00/02.0068, interní kód MU: Name: CEITEC - středoevropský technologický institut (Acronym: CEITEC)

Investor: Ministry of Education, Youth and Sports of the CR, CEITEC - Central European Institute of Technology, 1.1 European Centres of Excellence

• CZ.1.07/2.3.00/30.0030, interní kód MU: Name: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

• GBP302/12/G157, research and development project: Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

• MUNI/A/0855/2013, interní kód MU: Name: Rozsáhlé výpočetní systémy: modely, aplikace a verifikace III. (Acronym: FI MAV III.)

Investor: Masaryk University, Category A