a 2014

Regulation of H3K56ac during cell cycle in mammalian cells

STEJSKAL, Stanislav, Karel ŠTĚPKA, Karel STEJSKAL, Martina MATĚJKOVÁ, Pavel MATULA et. al.

Basic information

Original name

Regulation of H3K56ac during cell cycle in mammalian cells

Authors

STEJSKAL, Stanislav, Karel ŠTĚPKA, Karel STEJSKAL, Martina MATĚJKOVÁ, Pavel MATULA and Irena KRONTORÁD KOUTNÁ

Edition

Advances in Molecular and Cancer Biology, 2014

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Informatics

Keywords in English

H3K56ac; cancer cells; cell cycle
Změněno: 7/4/2015 13:58, Mgr. Stanislav Stejskal, Ph.D.

Abstract

V originále

Balance between the open and locked structure of chromatin is orchestrated by posttranslational histone modifications. Acetylation of histone H3 on core lysine 56 (H3K56ac) increases conformational entropy in alpha-N helix part and destabilize protein structure. This mechanism is distinct to N-tail acetylation that decreases electrochemical attraction between negatively charged DNA and basic histones. Nucleosomes containing H3K56ac show increased chromatin breathing and facilitate gene expression. However, the most H3K56ac research is connected with yeasts. Mammalian cells have a very low level of H3K56ac in comparison with N-tail histone acetylation, because cells do not contain histone acetyltransferases with high specificity to H3K56. However, recent studies reveal important role of H3K56ac in regulation of chromatin structure of the most transcription active genes. In yeasts, H3K56ac serves as a marker for distinguish maternal and daughter DNA strand needed for proper DNA repair. We are focused on study of H3K56ac role during mammalian cell cycle and its regulation.

Links

CZ.1.05/1.1.00/02.0068, interní kód MU
Name: CEITEC - středoevropský technologický institut (Acronym: CEITEC)
Investor: Ministry of Education, Youth and Sports of the CR, CEITEC - Central European Institute of Technology, 1.1 European Centres of Excellence
CZ.1.07/2.3.00/30.0030, interní kód MU
Name: Rozvoj lidských zdrojů pro oblast buněčné biologie
Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development
GBP302/12/G157, research and development project
Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
MUNI/A/0855/2013, interní kód MU
Name: Rozsáhlé výpočetní systémy: modely, aplikace a verifikace III. (Acronym: FI MAV III.)
Investor: Masaryk University, Category A