HP1B-dependent recruitment of UBF1 to irradiated chromatin
occurs simultaneously with CPDs

J 2014

HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

STIXOVÁ, Lenka, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Tereza HRUŠKOVÁ et. al.

Basic information

Original name

HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

Authors

STIXOVÁ, Lenka (203 Czech Republic), Petra SEHNALOVÁ (203 Czech Republic), Soňa LEGARTOVÁ (703 Slovakia), Jana SUCHÁNKOVÁ (203 Czech Republic), Tereza HRUŠKOVÁ (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Dmitry SOROKIN (643 Russian Federation, guarantor, belonging to the institution), Pavel MATULA (203 Czech Republic, belonging to the institution), Ivan RAŠKA (203 Czech Republic), Aleš KOVAŘÍK (203 Czech Republic), Jaroslav FULNEČEK (203 Czech Republic) and Eva BÁRTOVÁ (203 Czech Republic)

Edition

Epigenetics & Chromatin, BioMed Central, 2014, 1756-8935

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.333

RIV identification code

RIV/00216224:14330/14:00074061

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.1186/1756-8935-7-39

UT WoS

000347931500001

Keywords in English

DNA-damage response; DNA repair; Irradiation; Live-cell studies; Nucleolus; UBF1

Abstract

V originále

Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin. Transcription factor UBF1 also interacted directly with DNA inside the nucleolus but no interaction of UBF1 and DNA was confirmed outside the nucleolus, where UBF1 recruitment to DNA lesions appeared simultaneously with cyclobutane pyrimidine dimers; this occurrence was cell-cycle-independent. Conclusions. We propose that the simultaneous presence and interaction of UBF1 and HP1betta at DNA lesions is activated by the presence of cyclobutane pyrimidine dimers and mediated by the chromo shadow domain of HP1betta. This might have functional significance for nucleotide excision repair.

Links

CZ.1.07/2.3.00/30.0030, interní kód MU

Name: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

STIXOVÁ, Lenka, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Tereza HRUŠKOVÁ, Stanislav KOZUBEK, Dmitry SOROKIN, Pavel MATULA, Ivan RAŠKA, Aleš KOVAŘÍK, Jaroslav FULNEČEK and Eva BÁRTOVÁ. HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs (HP1B-dependent recruitment of UBF1 to 3 irradiated chromatin occurs simultaneously with 4 CPDs). Epigenetics & Chromatin. BioMed Central, 2014, vol. 7, No 39, p. 1-17. ISSN 1756-8935. Available from: https://dx.doi.org/10.1186/1756-8935-7-39.

@article{1205176,
   author = {Stixová, Lenka and Sehnalová, Petra and Legartová, Soňa and Suchánková, Jana and Hrušková, Tereza and Kozubek, Stanislav and Sorokin, Dmitry and Matula, Pavel and Raška, Ivan and Kovařík, Aleš and Fulneček, Jaroslav and Bártová, Eva},
   article_number = {39},
   doi = {http://dx.doi.org/10.1186/1756-8935-7-39},
   keywords = {DNA-damage response; DNA repair; Irradiation; Live-cell studies; Nucleolus; UBF1},
   language = {eng},
   issn = {1756-8935},
   journal = {Epigenetics & Chromatin},
   title = {HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs},
   volume = {7},
   year = {2014}
}

TY  - JOUR
ID  - 1205176
AU  - Stixová, Lenka - Sehnalová, Petra - Legartová, Soňa - Suchánková, Jana - Hrušková, Tereza - Kozubek, Stanislav - Sorokin, Dmitry - Matula, Pavel - Raška, Ivan - Kovařík, Aleš - Fulneček, Jaroslav - Bártová, Eva
PY  - 2014
TI  - HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
JF  - Epigenetics & Chromatin
VL  - 7
IS  - 39
SP  - 1-17
EP  - 1-17
PB  - BioMed Central
SN  - 17568935
KW  - DNA-damage response
KW  - DNA repair
KW  - Irradiation
KW  - Live-cell studies
KW  - Nucleolus
KW  - UBF1
N2  - Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin. Transcription factor UBF1 also interacted directly with DNA inside the nucleolus but no interaction of UBF1 and DNA was confirmed outside the nucleolus, where UBF1 recruitment to DNA lesions appeared simultaneously with cyclobutane pyrimidine dimers; this occurrence was cell-cycle-independent. Conclusions. We propose that the simultaneous presence and interaction of UBF1 and HP1betta at DNA lesions is activated by the presence of cyclobutane pyrimidine dimers and mediated by the chromo shadow domain of HP1betta. This might have functional significance for nucleotide excision repair.
ER  -

STIXOVÁ, Lenka, Petra SEHNALOVÁ, Soňa LEGARTOVÁ, Jana SUCHÁNKOVÁ, Tereza HRUŠKOVÁ, Stanislav KOZUBEK, Dmitry SOROKIN, Pavel MATULA, Ivan RAŠKA, Aleš KOVAŘÍK, Jaroslav FULNEČEK and Eva BÁRTOVÁ. HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs (HP1B-dependent recruitment of UBF1 to 3 irradiated chromatin occurs simultaneously with 4 CPDs). \textit{Epigenetics \&{} Chromatin}. BioMed Central, 2014, vol.~7, No~39, p.~1-17. ISSN~1756-8935. Available from: https://dx.doi.org/10.1186/1756-8935-7-39.

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