a 2014

The role of alternative splicing in auxin and cytokinin pathways

HRTYAN, Mónika, Eva ŠLIKOVÁ, Jan HEJÁTKO and Kamil RŮŽIČKA

Basic information

Original name

The role of alternative splicing in auxin and cytokinin pathways

Authors

HRTYAN, Mónika (348 Hungary, guarantor, belonging to the institution), Eva ŠLIKOVÁ (703 Slovakia, belonging to the institution), Jan HEJÁTKO (203 Czech Republic, belonging to the institution) and Kamil RŮŽIČKA (203 Czech Republic, belonging to the institution)

Edition

9th Tri-National Arabidopsis Meeting (TNAM) 2014, 2014

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/14:00077162

Organization unit

Faculty of Science

Keywords in English

alternative splicing; PIN; AHP6; cell plate
Změněno: 13/4/2015 10:30, Olga Křížová

Abstract

V originále

Alternative splicing is a tightly regulated process accompanying gene expression. It leads to multiple protein variants from a single primary transcipt. Transcriptome sequencing revealed that >60 % of Arabidopsis genes are alternatively spliced. This raises the question if all the identified isoforms have biologically functional relevance. Our aim is to answer this question for three members of auxin and cytokinin pathways: PIN4 (PINFORMED 4), PIN7 and AHP6 (ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6), where all of them are known to be processed into more alternatively spliced mRNAs. PIN4 and PIN7 are processed into two isoforms called a and b, respectively, which differ in their subcellular localization. Also two isoforms of AHP6 have been described, AHP6b and AHP6a. We found that both of them are expressed as stable proteins and are actively transported into nuclei.

Links

EE2.3.20.0189, research and development project
Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace