KUBÁŇ, Petr, Michal GREGUŠ, Eva POKOJOVÁ, Jana SKŘIČKOVÁ and František FORET. Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis. Journal of Chromatography A. AMSTERDAM: Elsevier, vol. 1358, September, p. 293-298. ISSN 0021-9673. doi:10.1016/j.chroma.2014.06.091. 2014.
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Basic information
Original name Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
Authors KUBÁŇ, Petr (203 Czech Republic, guarantor, belonging to the institution), Michal GREGUŠ (703 Slovakia, belonging to the institution), Eva POKOJOVÁ (203 Czech Republic), Jana SKŘIČKOVÁ (203 Czech Republic, belonging to the institution) and František FORET (203 Czech Republic, belonging to the institution).
Edition Journal of Chromatography A, AMSTERDAM, Elsevier, 2014, 0021-9673.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.169
RIV identification code RIV/00216224:14740/14:00074081
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.chroma.2014.06.091
UT WoS 000340979200035
Keywords in English Capillary electrophoresis; Contactless conductivity detection; Ions; Cystic fibrosis; Principal component analysis
Tags EL OK, kontrola MP, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 30/3/2015 09:07.
Abstract
A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed. (C) 2014 Elsevier B.V. All rights reserved.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GA13-21919S, research and development projectName: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.
Investor: Czech Science Foundation
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