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@article{1205561, author = {Kubáň, Petr and Greguš, Michal and Pokojová, Eva and Skřičková, Jana and Foret, František}, article_location = {AMSTERDAM}, article_number = {September}, doi = {http://dx.doi.org/10.1016/j.chroma.2014.06.091}, keywords = {Capillary electrophoresis; Contactless conductivity detection; Ions; Cystic fibrosis; Principal component analysis}, language = {eng}, issn = {0021-9673}, journal = {Journal of Chromatography A}, title = {Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis}, url = {http://www.sciencedirect.com/science/article/pii/S002196731401036X}, volume = {1358}, year = {2014} }
TY - JOUR ID - 1205561 AU - Kubáň, Petr - Greguš, Michal - Pokojová, Eva - Skřičková, Jana - Foret, František PY - 2014 TI - Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis JF - Journal of Chromatography A VL - 1358 IS - September SP - 293-298 EP - 293-298 PB - Elsevier SN - 00219673 KW - Capillary electrophoresis KW - Contactless conductivity detection KW - Ions KW - Cystic fibrosis KW - Principal component analysis UR - http://www.sciencedirect.com/science/article/pii/S002196731401036X L2 - http://www.sciencedirect.com/science/article/pii/S002196731401036X N2 - A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6. By using the target ion ratios rather than the concentrations of each individual ion combined with principal component analysis, the diagnosis of CF can be made more accurately and greatly reduce the number of false positive or negative results as is often the case when single ion (chloride) is analyzed. (C) 2014 Elsevier B.V. All rights reserved. ER -
KUBÁŇ, Petr, Michal GREGUŠ, Eva POKOJOVÁ, Jana SKŘIČKOVÁ and František FORET. Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis. \textit{Journal of Chromatography A}. AMSTERDAM: Elsevier, vol.~1358, September, p.~293-298. ISSN~0021-9673. doi:10.1016/j.chroma.2014.06.091. 2014.
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