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@proceedings{1205568,
author = {Šimara, Pavel and Tesařová, Lenka and Paďourová, Stanislava and Krontorád Koutná, Irena},
booktitle = {Advances in Molecular and Cancer Biology},
keywords = {hiPSCs; lentivirus; Sendai virus; episomal reprogramming},
language = {eng},
title = {Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods},
year = {2014}
}
TY - CONF
ID - 1205568
AU - Šimara, Pavel - Tesařová, Lenka - Paďourová, Stanislava - Krontorád Koutná, Irena
PY - 2014
TI - Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods
KW - hiPSCs
KW - lentivirus
KW - Sendai virus
KW - episomal reprogramming
N2 - Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfill this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during 7 years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSCs clones are highly preferable in terms of future clinical application. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human fibroblasts and CD34+ blood progenitors were used as a source cells and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions.
ER -
ŠIMARA, Pavel, Lenka TESAŘOVÁ, Stanislava PAĎOUROVÁ and Irena KRONTORÁD KOUTNÁ. Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods. In \textit{Advances in Molecular and Cancer Biology}. 2014.
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