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@inproceedings{1205945,
author = {Midlik, Adam and Sándor, Roman and Nosková, Kristýna and Dovrtělová, Gabriela and Juřica, Jan and Táborská, Eva and Peš, Ondřej},
address = {Brno},
booktitle = {CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS},
editor = {František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník},
keywords = {benzophenanthridine; metabolite; microsomes; mass spectrometry; liquid chromatography},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Brno},
isbn = {978-80-904959-2-0},
pages = {329-332},
publisher = {Institute of Analytical Chemistry AS CR},
title = {LIQUID CHROMATOGRAPHIC MASS SPECTROMETRIC DETECTION OF METABOLITES OF MINOR BENZO[C]PHENANTHRIDINE ALKALOIDS},
year = {2014}
}
TY - JOUR
ID - 1205945
AU - Midlik, Adam - Sándor, Roman - Nosková, Kristýna - Dovrtělová, Gabriela - Juřica, Jan - Táborská, Eva - Peš, Ondřej
PY - 2014
TI - LIQUID CHROMATOGRAPHIC MASS SPECTROMETRIC DETECTION OF METABOLITES OF MINOR BENZO[C]PHENANTHRIDINE ALKALOIDS
PB - Institute of Analytical Chemistry AS CR
CY - Brno
SN - 9788090495920
KW - benzophenanthridine
KW - metabolite
KW - microsomes
KW - mass spectrometry
KW - liquid chromatography
N2 - Quaternary benzo[c]phenanthridine alkaloids (QBAs) belong to a group of substances obtained from plants of families Papaveraceae, Fumariaceae and Rutaceae. While the major and commercially available sanguinarine (SA) and chelerythrine (CHE) have been involved in biological and pharmacological studies over recent years, metabolic data on minor QBAs including pentasubstituted sanguilutine (SL) and hexasubstituted macarpine (MA) are limited, due to their slow accumulation in plant material as they represent the end products in their respective biosynthetic pathways. One of the substantial parts of the pharmacokinetic and toxicity studies is determination of their metabolic fate. Enzymes of endoplasmatic reticulum, located on rat liver microsomes (RLMs), such as cytochrome P450, may serve as a simple in vitro model for metabolic studies. The metabolites can be analyzed by high performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (MS). Despite the very similar structure of QBAs, metabolic pathways may differ considerably. Results published on metabolites of SA and CHE indicate demethylation, hydroxylation and ring-opening reactions.
ER -
MIDLIK, Adam, Roman SÁNDOR, Kristýna NOSKOVÁ, Gabriela DOVRTĚLOVÁ, Jan JUŘICA, Eva TÁBORSKÁ a Ondřej PEŠ. LIQUID CHROMATOGRAPHIC MASS SPECTROMETRIC DETECTION OF METABOLITES OF MINOR BENZO[C]PHENANTHRIDINE ALKALOIDS. In František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník. \textit{CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. Brno: Institute of Analytical Chemistry AS CR, 2014. s.~329-332. ISBN~978-80-904959-2-0.
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