MIDLIK, Adam, Roman SÁNDOR, Kristýna NOSKOVÁ, Gabriela DOVRTĚLOVÁ, Jan JUŘICA, Eva TÁBORSKÁ and Ondřej PEŠ. LIQUID CHROMATOGRAPHIC MASS SPECTROMETRIC DETECTION OF METABOLITES OF MINOR BENZO[C]PHENANTHRIDINE ALKALOIDS. In František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník. CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS. Brno: Institute of Analytical Chemistry AS CR, 2014. p. 329-332. ISBN 978-80-904959-2-0.
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Basic information
Original name LIQUID CHROMATOGRAPHIC MASS SPECTROMETRIC DETECTION OF METABOLITES OF MINOR BENZO[C]PHENANTHRIDINE ALKALOIDS
Authors MIDLIK, Adam (703 Slovakia, belonging to the institution), Roman SÁNDOR (703 Slovakia, belonging to the institution), Kristýna NOSKOVÁ (203 Czech Republic, belonging to the institution), Gabriela DOVRTĚLOVÁ (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution) and Ondřej PEŠ (203 Czech Republic, guarantor, belonging to the institution).
Edition Brno, CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, p. 329-332, 4 pp. 2014.
Publisher Institute of Analytical Chemistry AS CR
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
RIV identification code RIV/00216224:14110/14:00082164
Organization unit Faculty of Medicine
ISBN 978-80-904959-2-0
UT WoS 000354547400108
Keywords in English benzophenanthridine; metabolite; microsomes; mass spectrometry; liquid chromatography
Tags EL OK, podil
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 26. 11. 2015 11:22.
Abstract
Quaternary benzo[c]phenanthridine alkaloids (QBAs) belong to a group of substances obtained from plants of families Papaveraceae, Fumariaceae and Rutaceae. While the major and commercially available sanguinarine (SA) and chelerythrine (CHE) have been involved in biological and pharmacological studies over recent years, metabolic data on minor QBAs including pentasubstituted sanguilutine (SL) and hexasubstituted macarpine (MA) are limited, due to their slow accumulation in plant material as they represent the end products in their respective biosynthetic pathways. One of the substantial parts of the pharmacokinetic and toxicity studies is determination of their metabolic fate. Enzymes of endoplasmatic reticulum, located on rat liver microsomes (RLMs), such as cytochrome P450, may serve as a simple in vitro model for metabolic studies. The metabolites can be analyzed by high performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (MS). Despite the very similar structure of QBAs, metabolic pathways may differ considerably. Results published on metabolites of SA and CHE indicate demethylation, hydroxylation and ring-opening reactions.
Links
LH12176, research and development projectName: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/0886/2013, interní kód MUName: Farmakodynamické, farmakokinetické a farmakogenetické přístupy v preklinické fázi výzkumu a vývoje léčiv (Acronym: FDN, FKK a FGEN studie)
Investor: Masaryk University, Category A
MUNI/A/0954/2013, interní kód MUName: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: BIOMET3)
Investor: Masaryk University, Category A
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