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@inproceedings{1206163,
author = {Rozman, Tomáš and Farka, Zdeněk and Havliš, Jan and Lubal, Přemysl and Farková, Marta},
address = {Brno},
booktitle = {CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS},
editor = {František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník},
keywords = {ANN; biogenic amines; dansyl chloride; HPLC},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Brno},
isbn = {978-80-904959-2-0},
pages = {377-380},
publisher = {Institute of Analytical Chemistry AS CR},
title = {Use of artificial neural networks for optimization of biogenic amines derivatization},
url = {http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf#page=378},
year = {2014}
}
TY - JOUR
ID - 1206163
AU - Rozman, Tomáš - Farka, Zdeněk - Havliš, Jan - Lubal, Přemysl - Farková, Marta
PY - 2014
TI - Use of artificial neural networks for optimization of biogenic amines derivatization
PB - Institute of Analytical Chemistry AS CR
CY - Brno
SN - 9788090495920
KW - ANN
KW - biogenic amines
KW - dansyl chloride
KW - HPLC
UR - http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf#page=378
N2 - Biogenic amines as biologically active molecules with potential toxicological dangers are often subject of interest in food analysis. Since these substances do not exhibit significant fluorescence nor absorbance, derivatization is necessary for analysis in combination with RP-HPLC-UV setup. The use of experimental design (ED) in combination with artificial neural networks (ANN) for optimization of dansyl derivatization conditions for seven biogenic amines is presented. Four key factors were studied – concentration of derivatization reagent, buffer pH, temperature and incubation time. With the use of ED-ANN approach, optimal conditions for dansyl chloride derivatization were found and experimentally confirmed.
ER -
ROZMAN, Tomáš, Zdeněk FARKA, Jan HAVLIŠ, Přemysl LUBAL and Marta FARKOVÁ. Use of artificial neural networks for optimization of biogenic amines derivatization. Online. In František Foret, Jana Křenková, Iveta Drobníková, Andras Guttman, Karel Klepárník. \textit{CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. Brno: Institute of Analytical Chemistry AS CR, 2014, p.~377-380. ISBN~978-80-904959-2-0.
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