Polymorphism 140A/G in lactotransferrin gene and its association with development of dental caries and gingivitis in children

BARTOŠOVÁ, Michaela, Petra BOŘILOVÁ LINHARTOVÁ, Tereza TREFNÁ, Jan VLAŽNÝ, Kristína MUSILOVÁ, Martina KUKLETOVÁ, Lubomír KUKLA and Lydie IZAKOVIČOVÁ HOLLÁ. Polymorphism 140A/G in lactotransferrin gene and its association with development of dental caries and gingivitis in children. In 6th International Student Medical Congress in Košice, 25th – 27th of June 2014. 2014. ISBN 978-80-8152-147-8.

Basic information

• Original name: Polymorphism 140A/G in lactotransferrin gene and its association with development of dental caries and gingivitis in children
• Name in Czech: Polymorfizmus 140 A/G v genu pro lactoferin a jeho vztah k rozvoji zubního kazu a gingivitídě u dětí
• Authors: BARTOŠOVÁ, Michaela (703 Slovakia, belonging to the institution), Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, belonging to the institution), Tereza TREFNÁ (203 Czech Republic), Jan VLAŽNÝ (203 Czech Republic), Kristína MUSILOVÁ (203 Czech Republic, belonging to the institution), Martina KUKLETOVÁ (203 Czech Republic, belonging to the institution), Lubomír KUKLA (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: 6th International Student Medical Congress in Košice, 25th – 27th of June 2014, 2014.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/14:00074103
• Organization unit: Faculty of Medicine
• ISBN: 978-80-8152-147-8
• ISSN: 1337-7817
• Keywords (in Czech): LTF; polymorfizmus; zubní kaz; gingivitída; děti
• Keywords in English: lactotransferrin; polymorphism; dental caries; gingivitis; children
• Tags: EL OK, podil

Abstract

Introduction: Dental caries and periodontal diseases are complex, multifactorial diseases and one of the most common illnesses worldwide. Recent evidence suggests a role of LTF in caries and periodontitis. The aim of this study was determine the frequency of the polymorphism 140A/G in lactotransferrin gene and its association with dental caries and gingivitis in children in Czech population. Material and Methods: School children aged 11 – 13 years were included in the study (N = 637). The subjects were divided into two groups: without caries (DMFT = 0) and with caries (DMFT1). The caries affected group was stratified as low (DMFT=1), moderate (2DMFT3) and high (DMFT4) caries experience. The level of gingivitis was rated according to gingival index (GI) and subjects were divided into the groups without (GI = 0) and with (GI1) gingivitis. The LTF rs1126478 (140A/G exon 2, Lys/Arg) genotypes were determined by polymerase-chain reaction (PCR) with restriction analysis by EarI enzyme. Results: Of 637 subjects, 155 (24.3%) were caries free children. There were no statistical significant differences between caries levels and allele or genotype distributions in the total cohort. When the caries affected group (N=482) was stratified as low (DMFT=1), moderate (2DMFT3) and high (DMFT4) caries experience, allele and genotype frequencies were similar among all subgroups. Gingivitis was diagnosed in 53.4% children. The difference in frequencies of alleles and genotypes between the group of healthy children and children with gingivitis was also not significant (p0.05). Conclusion: The polymorphism 140A/G in lactotransferrin gene was not associated with the susceptibility and severity of dental caries and gingivitis in the Czech population.

Abstract (in Czech)

Úvod: Zubní kaz a onemocnění závěsného aparátu zubu jsou světově nerozšířenější nemoci. V současné dostupné literatuře se poukazuje na funkci laktoferrinu v patogeneze vzniku zubního kazu a gingivitidy.Cílem studie bylo stanovit četnost polymorfizmu 140A/G v genu pro LTF a jeho vztah k rozvoji zubního kazu a gingivitidy u dětí v české populaci. Materiál a metody: Do studie bylo zařazeno 637 dětí ve věku 11 – 13let. Byli rozděleni do dvou skupin s kazem (KPE1) a bez kazu (KPE=0) Skupina s kazem byla dále rozdělena dle závažnosti do podskupin s nízkou (KPE=1), střední (2KPE3) a vysokou (KPE4) kazivostí. Stupeň zánětu dásní byl hodnocen gingiválním indexem (GI) a podle něho byli děti rozděleni do skupin s (GI1) a bez (GI = 0) gingivitídy. LTF rs1126478 (140A/G exon 2, Lys/Arg) genotypy byli determinované polymerázovou řetězcovou reakcí a restrikční analýzou enzymem EarI. Výsledky: 155 (24.3%) dětí bylo bez kazu. Nebyl prokázán statistiky významný rozdíl medzi skupinou dětí s kazem a bez kazu v genotypových ani alelických frekvencích. Na základě rozdělení dětí do podskupin podle závažnosti zubního kazu nebyl opět stanoven statisticky významný rozdíl.. Gingivitida byla diagnostikována u 53.4% dětí. Rozdíl v alelických a genotypových frekvencích opět nebyl statisticky významný (p>0.05). Závěr: Polymorfizmus 140A/G nebyl asociován se vznikem a závažností zubního kazu a gingivitídou u dětí v české populaci.

Links

• GB14-37368G, research and development project: Name: Centrum orofaciálního vývoje a regenerace

Investor: Czech Science Foundation

• MUNI/A/0951/2013, interní kód MU: Name: Onemocnění orofaciální soustavy - výzkum jejich etiopatogeneze, možností predikce, diagnostiky a terapie

Investor: Masaryk University, Category A

• NT11405, research and development project: Name: Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu

Investor: Ministry of Health of the CR