In-Cell NMR and EPR Spectroscopy of Biomacromolecules

J 2014

In-Cell NMR and EPR Spectroscopy of Biomacromolecules

HANSEL, Robert, Laura M. LUH, Ivan CORBERSKI, Lukáš TRANTÍREK, Volker DOTSCH et. al.

Basic information

Original name

In-Cell NMR and EPR Spectroscopy of Biomacromolecules

Authors

HANSEL, Robert (276 Germany), Laura M. LUH (276 Germany), Ivan CORBERSKI (276 Germany), Lukáš TRANTÍREK (203 Czech Republic, guarantor, belonging to the institution) and Volker DOTSCH (276 Germany)

Edition

Angewandte Chemie International Edition, Weinheim, Germany, Wiley-VCH Verlag GmbH & Co. 2014, 1433-7851

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 11.261

RIV identification code

RIV/00216224:14740/14:00074124

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/anie.201311320

UT WoS

000342760700004

Keywords in English

MAGNETIC-RESONANCE-SPECTROSCOPY; XENOPUS-LAEVIS OOCYTES; ELECTRON-PARAMAGNETIC-RESONANCE; PROTEIN-PROTEIN INTERACTIONS; HUMAN TELOMERIC DNA; DISTANCE MEASUREMENTS; ESCHERICHIA-COLI; LIVING CELLS; F-19 NMR; PHYSIOLOGICAL CONDITIONS

Abstract

V originále

The dream of cell biologists is to be able to watch biological macromolecules perform their duties in the intracellular environment of live cells. Ideally, the observation of both the location and the conformation of these macromolecules with biophysical techniques is desired. The development of many fluorescence techniques, including super-resolution fluorescence microscopy, has significantly enhanced our ability to spot proteins and other molecules in the crowded cellular environment. However, the observation of their structure and conformational changes while they attend their business is still very challenging. In principle, NMR and EPR spectroscopy can be used to investigate the conformation and dynamics of biological macromolecules in living cells. The development of in-cell magnetic resonance techniques has demonstrated the feasibility of this approach. Herein we review the different techniques with a focus on liquid-state in-cell NMR spectroscopy, provide an overview of applications, and discuss the challenges that lie ahead.

Links

GA13-28310S, research and development project

Name: Evolučně konzervované strukturní vlastnosti centromerické a telomerické DNA

Investor: Czech Science Foundation

Citovat

HANSEL, Robert, Laura M. LUH, Ivan CORBERSKI, Lukáš TRANTÍREK and Volker DOTSCH. In-Cell NMR and EPR Spectroscopy of Biomacromolecules. Angewandte Chemie International Edition. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co., 2014, vol. 53, No 39, p. 10300-10314. ISSN 1433-7851. Available from: https://dx.doi.org/10.1002/anie.201311320.

@article{1209991,
   author = {Hansel, Robert and Luh, Laura M. and Corberski, Ivan and Trantírek, Lukáš and Dotsch, Volker},
   article_location = {Weinheim, Germany},
   article_number = {39},
   doi = {http://dx.doi.org/10.1002/anie.201311320},
   keywords = {MAGNETIC-RESONANCE-SPECTROSCOPY; XENOPUS-LAEVIS OOCYTES; ELECTRON-PARAMAGNETIC-RESONANCE; PROTEIN-PROTEIN INTERACTIONS; HUMAN TELOMERIC DNA; DISTANCE MEASUREMENTS; ESCHERICHIA-COLI; LIVING CELLS; F-19 NMR; PHYSIOLOGICAL CONDITIONS},
   language = {eng},
   issn = {1433-7851},
   journal = {Angewandte Chemie International Edition},
   title = {In-Cell NMR and EPR Spectroscopy of Biomacromolecules},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract},
   volume = {53},
   year = {2014}
}

TY  - JOUR
ID  - 1209991
AU  - Hansel, Robert - Luh, Laura M. - Corberski, Ivan - Trantírek, Lukáš - Dotsch, Volker
PY  - 2014
TI  - In-Cell NMR and EPR Spectroscopy of Biomacromolecules
JF  - Angewandte Chemie International Edition
VL  - 53
IS  - 39
SP  - 10300-10314
EP  - 10300-10314
PB  - Wiley-VCH Verlag GmbH & Co.
SN  - 14337851
KW  - MAGNETIC-RESONANCE-SPECTROSCOPY
KW  - XENOPUS-LAEVIS OOCYTES
KW  - ELECTRON-PARAMAGNETIC-RESONANCE
KW  - PROTEIN-PROTEIN INTERACTIONS
KW  - HUMAN TELOMERIC DNA
KW  - DISTANCE MEASUREMENTS
KW  - ESCHERICHIA-COLI
KW  - LIVING CELLS
KW  - F-19 NMR
KW  - PHYSIOLOGICAL CONDITIONS
UR  - http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/anie.201311320/abstract
N2  - The dream of cell biologists is to be able to watch biological macromolecules perform their duties in the intracellular environment of live cells. Ideally, the observation of both the location and the conformation of these macromolecules with biophysical techniques is desired. The development of many fluorescence techniques, including super-resolution fluorescence microscopy, has significantly enhanced our ability to spot proteins and other molecules in the crowded cellular environment. However, the observation of their structure and conformational changes while they attend their business is still very challenging. In principle, NMR and EPR spectroscopy can be used to investigate the conformation and dynamics of biological macromolecules in living cells. The development of in-cell magnetic resonance techniques has demonstrated the feasibility of this approach. Herein we review the different techniques with a focus on liquid-state in-cell NMR spectroscopy, provide an overview of applications, and discuss the challenges that lie ahead.
ER  -

HANSEL, Robert, Laura M. LUH, Ivan CORBERSKI, Lukáš TRANTÍREK and Volker DOTSCH. In-Cell NMR and EPR Spectroscopy of Biomacromolecules. \textit{Angewandte Chemie International Edition}. Weinheim, Germany: Wiley-VCH Verlag GmbH \&{} Co., 2014, vol.~53, No~39, p.~10300-10314. ISSN~1433-7851. Available from: https://dx.doi.org/10.1002/anie.201311320.

Detailed Information on Publication Record