| ZÁBRADY, Matej, Vendula HRDINOVÁ, Bruno MÜLLER, Udo CONRAD, Jan HEJÁTKO a Lubomír JANDA. Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies. PLOS ONE : Public Library of Science. San Francisco: Public Library Science, 2014, roč. 9, č. 10, s. "nestránkováno", 9 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0109875. |
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@article{1210490,
author = {Zábrady, Matej and Hrdinová, Vendula and Müller, Bruno and Conrad, Udo and Hejátko, Jan and Janda, Lubomír},
article_location = {San Francisco},
article_number = {10},
doi = {http://dx.doi.org/10.1371/journal.pone.0109875},
keywords = {recombinant antibodies;immunomodulation;inhibition;protein-protein interactions;cytokinin signaling pathway},
language = {eng},
issn = {1932-6203},
journal = {PLOS ONE : Public Library of Science},
title = {Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies},
url = {http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875},
volume = {9},
year = {2014}
}
TY - JOUR
ID - 1210490
AU - Zábrady, Matej - Hrdinová, Vendula - Müller, Bruno - Conrad, Udo - Hejátko, Jan - Janda, Lubomír
PY - 2014
TI - Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies
JF - PLOS ONE : Public Library of Science
VL - 9
IS - 10
SP - "nestránkováno"
EP - "nestránkováno"
PB - Public Library Science
SN - 19326203
KW - recombinant antibodies;immunomodulation;inhibition;protein-protein interactions;cytokinin signaling pathway
UR - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875
L2 - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875
N2 - With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach. This selection method can serve as a prototype for developing unique recombinant antibodies able to interfere with virtually any biomolecule in the living cell.
ER -
ZÁBRADY, Matej, Vendula HRDINOVÁ, Bruno MÜLLER, Udo CONRAD, Jan HEJÁTKO a Lubomír JANDA. Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies. \textit{PLOS ONE : Public Library of Science}. San Francisco: Public Library Science, 2014, roč.~9, č.~10, s.~''nestránkováno'', 9 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0109875.
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