Targeted In Vivo Inhibition of Specific Protein–Protein
Interactions Using Recombinant Antibodies

J 2014

Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies

ZÁBRADY, Matej, Vendula HRDINOVÁ, Bruno MÜLLER, Udo CONRAD, Jan HEJÁTKO et. al.

Basic information

Original name

Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies

Authors

ZÁBRADY, Matej (703 Slovakia, belonging to the institution), Vendula HRDINOVÁ (203 Czech Republic, belonging to the institution), Bruno MÜLLER (756 Switzerland), Udo CONRAD (276 Germany), Jan HEJÁTKO (203 Czech Republic, belonging to the institution) and Lubomír JANDA (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLOS ONE : Public Library of Science, San Francisco, Public Library Science, 2014, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.234

RIV identification code

RIV/00216224:14740/14:00074131

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1371/journal.pone.0109875

UT WoS

000343941200081

Keywords in English

recombinant antibodies;immunomodulation;inhibition;protein-protein interactions;cytokinin signaling pathway

Abstract

V originále

With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach. This selection method can serve as a prototype for developing unique recombinant antibodies able to interfere with virtually any biomolecule in the living cell.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0189, research and development project

Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

GAP305/11/0756, research and development project

Name: Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (Acronym: HKRD-AHP)

Investor: Czech Science Foundation

GA13-25280S, research and development project

Name: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Czech Science Foundation

GA521/09/1699, research and development project

Name: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana

Investor: Czech Science Foundation, Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana

261863, interní kód MU

Name: BioNMR Facilities - Bio NMR (Acronym: BioNMR)

Investor: European Union, Capacities

Citovat

ZÁBRADY, Matej, Vendula HRDINOVÁ, Bruno MÜLLER, Udo CONRAD, Jan HEJÁTKO and Lubomír JANDA. Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies. PLOS ONE : Public Library of Science. San Francisco: Public Library Science, 2014, vol. 9, No 10, p. "nestránkováno", 9 pp. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0109875.

@article{1210490,
   author = {Zábrady, Matej and Hrdinová, Vendula and Müller, Bruno and Conrad, Udo and Hejátko, Jan and Janda, Lubomír},
   article_location = {San Francisco},
   article_number = {10},
   doi = {http://dx.doi.org/10.1371/journal.pone.0109875},
   keywords = {recombinant antibodies;immunomodulation;inhibition;protein-protein interactions;cytokinin signaling pathway},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE : Public Library of Science},
   title = {Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies},
   url = {http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1210490
AU  - Zábrady, Matej - Hrdinová, Vendula - Müller, Bruno - Conrad, Udo - Hejátko, Jan - Janda, Lubomír
PY  - 2014
TI  - Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies
JF  - PLOS ONE : Public Library of Science
VL  - 9
IS  - 10
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - Public Library Science
SN  - 19326203
KW  - recombinant antibodies;immunomodulation;inhibition;protein-protein interactions;cytokinin signaling pathway
UR  - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875
L2  - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875
N2  - With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach. This selection method can serve as a prototype for developing unique recombinant antibodies able to interfere with virtually any biomolecule in the living cell.
ER  -

ZÁBRADY, Matej, Vendula HRDINOVÁ, Bruno MÜLLER, Udo CONRAD, Jan HEJÁTKO and Lubomír JANDA. Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies. \textit{PLOS ONE : Public Library of Science}. San Francisco: Public Library Science, 2014, vol.~9, No~10, p.~''nestránkováno'', 9 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0109875.

Detailed Information on Publication Record