J 2014

Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies

ZÁBRADY, Matej, Vendula HRDINOVÁ, Bruno MÜLLER, Udo CONRAD, Jan HEJÁTKO et. al.

Basic information

Original name

Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies

Authors

ZÁBRADY, Matej (703 Slovakia, belonging to the institution), Vendula HRDINOVÁ (203 Czech Republic, belonging to the institution), Bruno MÜLLER (756 Switzerland), Udo CONRAD (276 Germany), Jan HEJÁTKO (203 Czech Republic, belonging to the institution) and Lubomír JANDA (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLOS ONE : Public Library of Science, San Francisco, Public Library Science, 2014, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.234

RIV identification code

RIV/00216224:14740/14:00074131

Organization unit

Central European Institute of Technology

DOI

UT WoS

000343941200081

Keywords in English

recombinant antibodies;immunomodulation;inhibition;protein-protein interactions;cytokinin signaling pathway

Tags

Tags

International impact, Reviewed
Změněno: 10/3/2015 19:08, Martina Prášilová

Abstract

V originále

With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach. This selection method can serve as a prototype for developing unique recombinant antibodies able to interfere with virtually any biomolecule in the living cell.

Links

ED1.1.00/02.0068, research and development project
Name: CEITEC - central european institute of technology
EE2.3.20.0189, research and development project
Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace
GAP305/11/0756, research and development project
Name: Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (Acronym: HKRD-AHP)
Investor: Czech Science Foundation
GA13-25280S, research and development project
Name: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development
Investor: Czech Science Foundation
GA521/09/1699, research and development project
Name: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana
Investor: Czech Science Foundation, Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana
261863, interní kód MU
Name: BioNMR Facilities - Bio NMR (Acronym: BioNMR)
Investor: European Union, Capacities